表皮干细胞对小鼠皮肤创面愈合的作用  

Effect of epidermal stem cells on skin wound healing in mice

作　　者：王海珊 林逢源 刘小瑜 刘馨竹 闫宇宁 郭笑 安丽萍 WANG Haishan;LIN Fengyuan;LIU Xiaoyu;LIU Xinzhu;YAN Yuning;GUO Xiao;AN Liping(Department of Microbiology and Biochemical Pharmacy Research,School of Pharmacy,Beihua University,Jilin 132013,China)

机构地区：[1]北华大学药学院微生物与生化药学教研室,吉林吉林132013

出　　处：《中国比较医学杂志》2023年第4期57-62,共6页Chinese Journal of Comparative Medicine

基　　金：吉林省科技厅项目(20200404126YY,20210204158YY)。

摘　　要：目的 体外分离培养表皮干细胞(epidermal stem cells, ESCs),通过将ESCs移植皮肤损伤模型小鼠,探讨ESCs对小鼠皮肤创面愈合的作用。方法 取包皮环切术后的皮肤组织,采用黏附型Ⅳ型胶原分离培养ESCs, 3T3细胞作为饲养层分选ESCs。采用背侧创伤法建立小鼠皮肤损伤模型,随机分为空白对照组、模型组和点注射细胞组。治疗第0、3、7、11、15天拍照记录并计算创面愈合率。治疗第16天,测定小鼠组织中SOD、GSH、GSH-Px、MDA含量;ELISA测定小鼠血清中IL-10、TGF-β和TNF-α含量;HE染色观察创面皮肤组织病理学改变。结果 治疗第7、11天,点注射细胞组创面面积均低于模型组(P<0.05),第15天点注射细胞组伤口几乎愈合(98.08%±1.77%)。与模型组相比,点注射细胞组小鼠组织中SOD、GSH、GSH-Px含量显著升高(P<0.01),MDA含量显著降低(P<0.01);ELISA结果显示,与模型组相比,点注射细胞组小鼠血清中IL-10和TGF-β含量升高(P<0.05)、TNF-α含量降低(P<0.05)。HE染色结果表明,与模型组相比,点注射细胞组炎症细胞浸润减少,有较多的毛囊和胶原生成。结论 ESCs可以加速创面上皮化,有效促进伤口创面愈合。Objective In vitro isolation and culture of epidermal stem cells(ESCs)and transplantation of ESCs into skin injury model mice to explore their effect on skin wound healing.Methods Circumcised skin tissue was taken,and ESCs were separated and cultured by adherent type IV collagen;3T3 cells were used as the feeder layer to sort ESCs.A mouse skin injury model was established by dorsal trauma method,and mice were randomly divided into a normal control group,model group,and spot injection cell group.The wound healing rates were recorded on days 0,3,7,11,and 15 of treatment.On the 16th day of treatment,we determined the contents of SOD,GSH,GSH-Px,and MDA in the tissues.ELISA was used to determine IL-10,TGF-β,and TNF-αlevels in the serum.HE staining was used to observe histopathological changes in the skin wounds.Results On the 7th and 11th days of treatment,the wound area in the spot injection cell group group was smaller than that in the model group(P<0.05),and the spot injection cell group’s wounds were almost healed on the 15th day(98.08%±1.77%).Compared with the model group,the spot injection cell group’s contents of SOD,GSH,GSH-Px were significantly increased(P<0.01),and MDA was significantly reduced(P<0.01);the ELISA result showed that the serum contents of IL-10 and TGF-βwere increased(P<0.05)and TNF-αcontent had declined in the spot injection cell group(P<0.05).The result of HE staining showed that,compared with model group,the spot injection cell group had reduced infiltration of inflammatory cells and more hair follicles and collagen.Conclusions ESCs can accelerate wound epithelialization and effectively promote wound healing.

关 键 词：表皮干细胞 创面愈合 炎症 氧化应激 

分 类 号：R-33[医药卫生]