乙型肝炎转基因小鼠品系C57-TgN(HBV adr2.0)SMMU的生物学特征  

Biological characters of hepatitis B virus transgenic mice strain C57-TgN(HBV adr2. 0)SMMU

作　　者：訾晓渊[1] 姚玉成[1] 熊俊[1] 金艳花[1] 叶煦亭[1] 李建秀[1] 刘红[1] 朱海英[1] 王新民[1] 倪文君[1] 胡以平[1] 余宏宇 丛文铭[3] 

机构地区：[1]第二军医大学基础医学部细胞生物学教研室,上海200433 [2]长征医院病理科 [3]东方肝胆外科医院病理科

出　　处：《第二军医大学学报》2002年第11期1179-1183,共5页Academic Journal of Second Military Medical University

基　　金：国家"九五"攻关项目(TJ99-LA01);国家自然科学 基金资助项目(39670811);上海市科学技术发展基金项目 (994919033).

摘　　要：目的:评价乙肝转基因小鼠品系C57-TgN(HBV adr2.O)SMMU生物学特征的稳定性。方法:以F5代乙肝转基因小鼠C57-TgN(HBV adr2.O)SMMU为研究对象,采用基因组DNA PCR、血清ELISA检测、Western印迹分析、免疫组织化学、血清DNA PCR、透射电镜和H-E染色的方法分析HBV基因在转基因小鼠中的整合、表达、复制和组织学变化。结果:F1代乙肝转基因小鼠基因组中稳定整合有HBV基因,肝组织中可检测到HBsAg、HBcAg和X蛋白3种病毒蛋白,血清中HB-sAg和HBeAg的表达率分别为19.54％和3.39％,且在血清和肝组织中存在病毒DNA和病毒样颗粒;长期的病毒DNA整合、表达和复制可以引起转基因小鼠肝、肺等组织的病理性损伤。结论:乙肝转基因小鼠品系C57-TgN(HBV adr2.O:)SMMU具有基因组中稳定整合病毒DNA、血清和肝组织中有病毒蛋白表达和病毒复制的特征,并具有一定的组织学变化,作为生物医药研究的实验动物模型具有推广应用价值。Objective:To evaluate the biological characters of C57-TgN(HBV adr2.0)SMMU transgenic mice. Methods: Integration,expression,replication and histology change of hepatitis B virus gene in F6 transgenic mice were estimated by ge-nomic DNA PCR,Western blotting,ELISA,immunohistochemistry,serum DNA PCR,transmission electron microscopy and H-E staining. Results: Hepatitis B virus gene was integrated into F6 C57-TgN(HBV adr2. 0)SMMU transgenic mice and expressed HBsAg,HBcAg and X protein in liver tissue. HBsAg and HBeAg were expressed in serum of 19. 54% and 3. 39% F6 transgenic mice. Hepatitis B virus were replicated in serum and liver tissue of transgenic mice. Long-term integration,expression and replication of hepatitis B virus gene induced pathological lesion of transgenic mice liver and lung. Conclusion: C57-TgNCHBV adr2. 0)SMMU transgenic mice line has the biological characters including integration of hepatitis B virus gene into genomic DNA,expression and replication of hepatitis B virus gene in serum and liver, and histological change in liver and lung. It is a valuable animal system to study pathogenesis, treatment and prevention of hepatitis B virus.

关 键 词：乙型肝炎病毒 转基因小鼠 免疫组织化学 病理学 

分 类 号：R373.21[医药卫生—病原生物学]