A multi-layer genome mining and phylogenomic analysis to construct efficient and autonomous efflux system for medium chain fatty acids  

作　　者：Hu Peng Lin Zhou Xuguo Duan Zhaojun Wang Zhaoshi Wang Mo Xia Mingsheng Dong Junjun Wu 

机构地区：[1]College of Food Science and Technology,Nanjing Agricultural University,Nanjing,Jiangsu 210095,China [2]Department of Food Science and Technology,College of Light Industry and Food Engineering,Nanjing Forestry University,Nanjing 210037,China [3]School of Biotechnology,Jiangnan University,1800 Lihu Road,Wuxi 214122,China

出　　处：《Food Materials Research》2022年第1期139-152,共14页食材研究（英文）

基　　金：supported by Natural Science Foundation of Jiangsu Province (BK20202002);Excellent Youth Foundation of Jiangsu Scientific Committee (BK20211526);Jiangsu Agricultural Science and Technology Innovation Fund (SCX (20)3332);National Natural Science Foundation of China (No.31972060);Fellowship of China Postdoctoral Science Foundation (2020T130305);Fundamental Research Funds for the Central Universities (KYGD202003);China Postdoctoral Science Foundation (2018M640491);Postdoctoral Research Funding of Jiangsu Province (2018K030B);the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

摘　　要：Medium-chain fatty acids(MCFAs)are important components for food,pharmaceutical and fuel industries.Nevertheless,engineering microorganisms to produce MCFAs often induces toxicity and stresses towards host strains,which could be alleviated via accelerating the export of MCFAs from cells.However,current secretory systems are inefficient and require inducible promoters.Here,a multi-layer genome mining and phylogenomic analysis was developed to identify efficient efflux transporters.Firstly,based on the genomic mining of 397 strains throughout various representative species,the evolutionary history of efflux transporters was recapitulated,and further experimental analysis revealed that acrE from Citrobacter exhibited the best performance.Secondly,according to the further mining of 797 Citrobacter genomes and 1084 Escherichia genomes,a detailed phylogenomic analysis of efflux transporter-centric genomic vicinities was performed.This led to the identification of efficient efflux pump combination acrE and acrF.These efflux pumps were then combined with the quorum-sensing circuit from Enterococcus faecalis to regulate MCFA efflux in an autonomous manner,which achieved a 4.9-fold boost in MCFA production and firstly demonstrated the efficient and autonomous efflux pump specially for MCFAs.The integrative omics technologies described here are enabling the utilization of the increasingly large database and the effective mining of target gene diversities.

关 键 词：mining AUTONOMOUS ANALYSIS 

分 类 号：TS202.1[轻工技术与工程—食品科学]