火龙果种子无菌苗组培育苗技术体系的建立  

Establishment of Breeding Technology System for Pitaya Sterile Seedlings Tissue Culture

作　　者：叶维雁 潘如军 汪艳华[2] 黄秋伟[1] 敖苏[3,4] 袁洋 朱朝华 刘贺贺[5] YE Weiyan;PAN Rujun;WANG Yanhua;HUANG Qiuwei;AO Su;YUANYang;ZHU Chaohua;LIU Hehe(Guangxi Subtropical Crops Research Institute,Nanning,Guangxi 530001,China;Guangxi Mountainous Area Comprehensive Technology Development Center,Nanning,Guangxi 530012,China;Hainan University,Haikou,Hainan 570228,China;Tropical Plants Isolation and Quarantine Center of Haikou Customs,Haikou,Hainan 570311,China;Tongren Agriculture Science and Technology Park Management Center,Tongren,Guizhou 554300,China)

机构地区：[1]广西壮族自治区亚热带作物研究所,广西南宁530001 [2]广西山区综合技术开发中心,广西南宁530012 [3]海南大学,海南海口570228 [4]海口海关热带植物隔离检疫中心,海南海口570311 [5]铜仁市农业科技园区管理中心,贵州铜仁554300

出　　处：《农业研究与应用》2023年第1期33-38,共6页Agricultural Research and Application

基　　金：广西农业科学院科技发展基金资助项目(桂农科2021JM124,桂农科2020YM134);广西重点研发计划(桂科AB16380076);南宁市重点研发计划(20172011-2);南宁市重大科技专项(20162008)。

摘　　要：以火龙果种子在无菌条件下萌发的幼苗作为外植体供体,子叶切段作为初代培养接种材料,通过调节不同外源激素及其浓度组合,选择最佳初代培养基、继代培养基和生根培养基,对建立火龙果组培育苗技术体系进行研究。结果表明:(1)种子经预处理后,用75%酒精浸泡10 s,再用0.1%Hg Cl_(2)浸泡5min的消毒效果最好,污染率为11.67%,萌发率为92.48%;(2)初代培养的最适培养基为MS+6-BA 2.0 mg·L^(-1)+NAA 0.2 mg·L^(-1)+蔗糖30 g·L^(-1),不定芽诱导率高达98.89%;(3)继代培养的最适培养基为MS+6-BA1.5 mg·L^(-1)+NAA 0.2 mg·L^(-1)+蔗糖30g·L^(-1),不定芽增殖系数达到6.52,不定芽粗壮,长势旺盛;(4)最佳生根培养基为1/2MS+NAA 0.5 mg·L^(-1)+IBA 0.1 mg·L^(-1)+蔗糖30 g·L^(-1)+AC 500 mg·L^(-1),生根率达98.89%,生根数为6.40条;(5)生根苗移栽30 d后成活率达97%。本研究以种子获取无菌苗作为外植体建立了火龙果组培技术体系,较茎段作为外植体有污染低,取材方便的优点。Taking pitaya seedlings sprouted under sterile conditions as explant donors,and cotyledon cuts as inoculation material of primary culture,the optimal primary culture medium,subculture culture medium and rooting medium were selected by adjusting combinations of different exogenous hormones and their concentrations,so as to study how to establish breeding technology system of pitaya tissue culture.The results showed that:(1)Immersing the seeds in 75%alcohol for 10 s and then in 0.1%HgCl_(2)for 5 min after pretreatment could gain optimal disinfection,with a contamination rate of 11.67%,and germination rate 92.48%;(2)the optimal medium for primary culture was MS+6-BA 2.0mg⋅L^(-1)+NAA 0.2mg⋅L^(-1)+sucrose 30g⋅L^(-1),leading to adventitious bud induction rate up to 98.89%;(3)MS+6-BA 1.5 mg⋅L^(-1)+NAA 0.2 mg⋅L^(-1)+sucrose 30g⋅L^(-1)was the most suitable for the subculture,leading to multiplication coefficient of 6.52,stout adventitious buds that grew vigorously;(4)the optimal rooting medium was 1/2 MS+NAA 0.5 mg⋅L^(-1)+IBA 0.1 mg⋅L^(-1)+sucrose 30g⋅L^(-1)+AC 500mg⋅L^(-1),leading to a rooting rate of 98.89%and rooting number 6.40;(5)survival rate of rooting plantlets reached 97%30d after transplantion.In this study,the tissue culture technology systemof pitayawas established by using sterile seedlings as explant donors,and compared with using stemsegments as explants,it has the advantages of lowpollution and convenient sampling.

关 键 词：火龙果 种子 组培 快速繁殖 

分 类 号：S667.9[农业科学—果树学]