微生物协同硫铁矿强化去除水中锑酸盐的性能研究  

Enhancement of antimonate removal from water by combining activated sludge and pyrite

作　　者：何巧冲 赵光华 郑旭宏 曹改改 王一朵 万东锦[1] HE Qiaochong;ZHAO Guanghua;ZHENG Xuhong;CAO Gaigai;WANG Yiduo;WAN Dongjin(School of Environmental Engineering,Henan University of Technology,Zhengzhou 450001)

机构地区：[1]河南工业大学环境工程学院,郑州450001

出　　处：《环境科学学报》2023年第5期154-162,共9页Acta Scientiae Circumstantiae

基　　金：国家自然科学基金青年基金(No.42207066);河南省教育厅科学技术研究重点项目基础研究计划(No.21A610004);河南工业大学博士科研启动基金(No.2019039)。

摘　　要：水体重金属锑(Sb)污染受到越来越多的关注.本研究旨在利用微生物和硫铁矿协同强化去除水中锑酸盐(Sb(Ⅴ)),实验对比了硫铁矿和硫化亚铁在非生物和生物反应体系中对Sb(Ⅴ)的去除效果.结果表明,在非生物(未接种活性污泥)反应体系中,硫铁矿和硫化亚铁对Sb(Ⅴ)的去除效果随运行时间延长而逐渐下降,且在第10个循环硫铁矿对Sb(Ⅴ)的去除率仅为14.5%;硫化亚铁相对硫铁矿有较高的Sb(Ⅴ)去除效果.在微生物作用下,硫铁矿和硫化亚铁具有较稳定的Sb(Ⅴ)去除效果,且硫铁矿对Sb(Ⅴ)的去除率高于硫化亚铁.通过X射线光电子能谱(XPS)分析证明微生物可加速Sb(Ⅴ)转化为Sb(Ⅲ),同时生成的Fe(Ⅱ)/Fe(Ⅲ)会促进Sb(Ⅲ)在硫铁矿上的富集沉淀,进而强化Sb从水体中去除.利用Illumina高通量测序揭示了除锑过程中微生物群落结构的时间演替规律,证明Thiobacillus和Sulfurimonas菌属为微生物协同硫铁矿去除Sb(Ⅴ)的优势菌属.研究结果为Sb(Ⅴ)废水修复提供了一种稳定高效的方法.The pollution of heavy metal antimony(Sb)in water has received more and more attention.This study aimed to enhance the removal of antimonate(Sb(Ⅴ))from water by combining microorganisms and pyrite.The removal performance of Sb(Ⅴ)was compared using pyrite and ferrous sulfide in abiotic(non-inoculated activated sludge)and biological reaction systems,respectively.Results show that in the reaction system without activated sludge inoculation,the removal efficiencies of Sb(Ⅴ)by pyrite and ferrous sulfide gradually decreased over time,and at the 10th cycle,the Sb(Ⅴ)removal efficiency by pyrite was only 14.5%.Compared with pyrite,ferrous sulfide had higher Sb removal efficiency in abiotic system.In the biological reaction systems,pyrite and ferrous sulfide had relatively stable Sb(Ⅴ)removal performance,and the removal efficiency of Sb by pyrite was higher than that by ferrous sulfide.X-ray photoelectron spectroscopy(XPS)analysis revealed that microorganisms promoted the conversion of Sb(Ⅴ)to Sb(Ⅲ),and the generated Fe(Ⅱ)/Fe(Ⅲ)promoted the enrichment and precipitation of Sb(Ⅲ)on pyrite;thus enhancing the Sb removal from water.The temporal succession of microbial community structure in the antimony removal process was revealed by Illumina high-throughput sequencing.In addition,it was revealed that Thiobacillus and Sulfurimonas were the dominant genus and may participate in the Sb(Ⅴ)reduction.This study provides a stable and efficient treatment method for Sb(Ⅴ)wastewater remediation.

关 键 词：硫铁矿 硫化亚铁 锑酸盐 微生物还原 优势菌属 

分 类 号：X703[环境科学与工程—环境工程]