老年性痴呆患者血清miR-375、SP1水平与认知功能的相关性  

Correlation between serum miR-375 and SP1 levels and cognitive function in patients with senile dementia

作　　者：夏君燕[1] 李亚男 章隽[1] 高永红[1] XIA Junyan;LI Yanan;ZHANG Jun;GAO Yonghong(Department of Geriatric Medicine,Beijing Aerospace General Hospital,Fengtai 100076,China)

机构地区：[1]北京航天总医院老年医学科,北京丰台100076

出　　处：《东南大学学报（医学版）》2023年第2期257-266,共10页Journal of Southeast University(Medical Science Edition)

基　　金：北京市丰台区卫生健康系统科研项目(2020-83)。

摘　　要：目的:检测老年性痴呆患者血清微小RNA-375(miR-375)、特异性蛋白1(SP1)表达水平,探讨其与认知功能的相关性。方法:选取2021年4月至2022年4月在本院就诊的老年性痴呆患者120例纳入疾病组,根据临床痴呆评定量表(CDR)评定患者病情严重程度,选取同期在本院进行健康体检的志愿者120例纳入对照组。采用实时荧光定量PCR(qRT-PCR)法检测所有受试者血清miR-375、SP1 mRNA表达水平。采用简易精神状态量表(MMSE)评估所有受试者的认知功能。比较不同病情严重程度患者血清miR-375、SP1 mRNA表达水平及MMSE评分。采用Pearson相关分析疾病组血清miR-375与SP1 mRNA表达水平的相关性,Spearman相关分析两者与MMSE评分的相关性。采用多因素Logistic回归分析影响老年性痴呆发生的危险因素。采用受试者工作特征(ROC)曲线分析血清miR-375、SP1 mRNA表达水平和MMSE评分对老年性痴呆患者病情进展为重度的预测价值。采用双荧光素酶报告基因实验检测miR-375与SP1的关系。体外培养人神经母细胞瘤细胞SH-SY5Y,通过β-淀粉样蛋白肽25-35(Aβ25-35)诱导构建老年性痴呆体外细胞模型,观察过表达miR-375对Aβ25-35诱导的SH-SY5Y细胞中SP1 mRNA和蛋白表达以及细胞活力、凋亡率的影响。结果:疾病组血清miR-375表达水平、MMSE评分显著低于对照组,SP1 mRNA表达水平显著高于对照组(P<0.05)。重度组、中度组血清miR-375表达水平、MMSE评分显著低于轻度组,SP1 mRNA表达水平显著高于轻度组(P<0.05);重度组血清miR-375表达水平、MMSE评分显著低于中度组,SP1 mRNA表达水平显著高于中度组(P<0.05)。Pearson相关分析显示,疾病组血清miR-375与SP1 mRNA表达水平呈负相关(r=-0.627,P<0.05);Spearman相关分析显示,血清miR-375表达水平与MMSE评分呈正相关(r=0.664,P<0.05),血清SP1 mRNA表达水平与MMSE评分呈负相关(r=-0.472,P<0.05)。多因素Logistic回归分析显示,低MMSE评分、miR-375�Objective:To detect the expression levels of serum microRNA-375(miR-375)and specific protein 1(SP1)in patients with senile dementia,and explore their correlation with cognitive function.Methods:120 patients with senile dementia who were treated in our hospital from April 2021 to April 2022 were selected as the disease group,and the severity of the disease was evaluated according to the Clinical Dementia Rating Scale(CDR).In addition,120 volunteers who underwent physical examination in our hospital at the same time were regarded as the control group.Serum miR-375 and SP1 mRNA expression levels in all subjects were detected by real-time quantitative PCR(qRT-PCR).Cognitive function in all subjects was assessed by Mini-Mental State Examination(MMSE).Serum miR-375 and SP1 mRNA expression levels and MMSE scores of patients with different disease severity were compared.Correlation between serum miR-375 and SP1 mRNA expression levels in the disease group was analyzed by Pearson correlation,and correlation between the two and the MMSE score was analyzed by Spearman correlation.Risk factors affecting the occurrence of senile dementia were analyzed by multivariate Logistic regression.Predictive value of serum miR-375,SP1 mRNA expression level and MMSE score on disease progressing to severe in patients with senile dementia was analyzed by receiver operating characteristic(ROC)curve.Relationship between miR-375 and SP1 was analyzed by dual-luciferase reporter gene assay.Human neuroblastoma cell SH-SY5Y was cultured in vitro and senile dementia cell model was constructed byβ-amyloid peptide 25-35(Aβ25-35)induction,and the effects of overexpression of miR-375 on the expression of SP1 mRNA and protein,cell viability and apoptosis rate in SH-SY5Y cells induced by Aβ25-35 were observed.Results:Serum miR-375 expression level and MMSE score in the disease group were significantly lower than those in the control group,and SP1 mRNA expression level was significantly higher than that in the control group(P<0.05).Serum miR-375 expres

关 键 词：老年性痴呆 微小RNA-375 特异性蛋白1 认知功能 相关性 

分 类 号：R749.6[医药卫生—神经病学与精神病学]