痛泻要方联合穴位埋线对肝郁脾虚型溃疡性结肠炎大鼠5-HT和SP的影响  

Effect of Tongxie Yaofang combined with acupoint catgut embedding on 5-HT and SP in ulcerative colitis rats with liver stagnation and spleen deficiency

作　　者：刘雅璇 董慧 段迤腾 王燚霈[2] 朱莹 LIU Yaruan;DONG Hui;DUAN Yiteng;WANG Yipei;ZHU Ying(Department of Spleen and Stomach Diseases,the First Hospital of Hunan University of Chinese Medicine,Changsha,410007,China;Department of Gastroenterology,the Second Affiliated Hospital of Hunan University of Chinese Medicine)

机构地区：[1]湖南中医药大学第一附属医院脾胃病科,长沙410007 [2]湖南中医药大学第二附属医院消化内科

出　　处：《中国中西医结合消化杂志》2023年第5期359-366,共8页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

基　　金：国家自然科学基金(No:81874466);湖南省教育厅科学研究项目(No:20A378)。

摘　　要：目的:观察痛泻要方联合穴位埋线对肝郁脾虚型溃疡性结肠炎大鼠结肠、海马组织中5-羟色胺(5-hydroxytryptamine, 5-HT)、神经递质P物质(neurotransmitter substance P,SP)的影响,并探讨其作用机制。方法:将48只SPF级SD雄性大鼠随机分为空白组(12只)、造模组(36只)。采用2,4,6-三硝基苯磺酸/乙醇混合剂灌肠+束缚+饮食失节+夹尾刺激制备肝郁脾虚型溃疡性结肠炎模型。将成模大鼠随机分为模型组、痛泻要方组、痛泻要方联合穴位埋线组;痛泻要方联合埋线组予以痛泻要方汤剂灌胃,连续14 d,同时于足三里、天枢、肝俞、期门、脾俞、大肠俞、膈俞各穴位处埋线,每7 d进行1次,共2次;痛泻要方组予痛泻要方汤剂灌胃;空白组和模型组予等体积纯净水进行灌胃,1次/d,干预14 d。采用苏木精-伊红染色观察结肠组织病理变化,免疫组织化学法检测大鼠结肠、海马组织中5-HT、SP的表达;ELISA法检测大鼠血清、海马组织中5-HT、SP的表达;RT-qPCR法检测结肠组织中肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、IL-6、IL-1β、IL-23含量的变化。结果:与空白组相比,余3组血清、海马及结肠组织中5-HT、SP的表达量明显增加(P<0.05),结肠组织中TNF-α、IL-6、IL-1β、IL-23的含量显著升高(P<0.05);与模型组比较,痛泻要方组、痛泻要方联合穴位埋线组血清、海马及结肠组织中5-HT、SP的表达量明显减少(P<0.05),结肠组织中TNF-α、IL-6、IL-1β、IL-23的含量显著降低(P<0.05)。结论:痛泻要方联合穴位埋线可以显著下调大鼠血清、海马及结肠组织中5-HT、SP的表达水平,并抑制炎性因子的表达,达到对肝郁脾虚型溃疡性结肠炎大鼠的治疗作用。Objective: To observe the effects of Tongxie Yaofang combined with acupoint catgut embedding on 5-hydroxytryptamine(5-HT) and neurotransmitter substance P(SP) in colonic and hippocampal tissues of rats with ulcerative colitis of liver depression and spleen deficiency, and to explore its mechanism. Methods: Totally 48 SPF SD male rats were randomly divided into blank group(12 rats) and modeling group(36 rats). The ulcerative colitis models of liver depression and spleen deficiency pattern is established by 2, 4, 6-tritrobenzene sulfonic acid/ethanol mixture enema combined with physical restraint, disorder diet and tail-clipping. The successfully established rat models were randomly divided into model group, Tongxie Yaofang group and Tongxie Yaofang combined with embedding group. The Tongxie Yaofang group and the combination group were treated with Tongxie Yaofang decoction by gavage for 14 days, and the combination group was also treated with acupoint embedding at "Zusanli", "Tianshu", "Ganshu", "Qimen", "Pishu", "Dachangshu" and "Geshu",seven days/time, a total of two times;and the blank group and model group were given same amount of purified water by gavage for 14 days. HE staining was used to observe the pathological changes of colon tissue and the expression of 5-HT and SP in colonic and hippocampal tissues of rats was detected by immunohistochemistry. 5-HT and SP in serum and hippocampal tissue were detected by ELISA, and tumor necrosis factor-α(TNF-α), interleukin-6(IL-6), IL-1β and IL-23 in colon tissue were detected by Real-time quantitative Polymerase Chain Reaction(RT-qPCR). Results: Compared with blank group, the expression levels of 5-HT and SP in serum, hippocampus and colon tissues were significantly increased(P<0.05), and the contents of TNF-α, IL-6, IL-1β and IL-23 in colon tissues were significantly increased(P<0.05). Compared with model group, the expressions of 5-HT and SP in serum, hippocampus and colon tissue of Tongxie Yaofang group and Tongxie Yaofang combined with acupoint catgut grou

关 键 词：溃疡性结肠炎 穴位埋线 痛泻要方 5-羟色胺 神经递质P物质 脑肠轴 肿瘤坏死因子-α 

分 类 号：R574.62[医药卫生—消化系统]