基于微流控技术的数字PCR的发展及其应用  

Development and Application of Digital PCR Based on Microfluidic Technology

作　　者：田昌敏 杨祥良[1] 杨海[1] Tian Changmin;Yang Xiangliang;Yang Hai(National Engineering Research Center for Nanomedicine,College of Life Science and Technology,Huazhong University of Science and Technology,Wuhan 430074,China)

机构地区：[1]华中科技大学生命科学与技术学院、国家纳米药物工程技术研究中心,武汉430074

出　　处：《微纳电子技术》2023年第4期496-507,共12页Micronanoelectronic Technology

基　　金：国家自然科学基金面上项目(62275093)。

摘　　要：作为一种新兴的扩增检测技术,基于微流控的数字聚合酶链式反应(PCR)有着高通量、高灵敏度及高耐受性等优势,因而得到了研究者的广泛关注,其相关技术也在不断的发展中。综述了基于微流控的数字PCR的研究进展,重点讨论了基于微流控的数字PCR的液滴打印技术、多层微流控芯片技术、微珠技术、聚二甲基硅氧烷(PDMS)技术等的不同实现方式。介绍了基于微流控的数字PCR在定量检测、精准分子诊断、肿瘤个性化诊断以及食品安全检测中的应用。最后,对基于微流控的数字PCR技术目前存在的不足和问题进行了阐述,并对其未来的研究方向和发展趋势进行了展望。As a new type of amplification and detection technology,digital polymerase chain reaction(PCR)based on microfluidic has the advantages of high throughput,high sensitivity and high tolerance,so it has been widely concerned by researchers,and its related technologies are also in constant development.The research progress of digital PCR based on microfluidic is reviewed,the different implementation ways of technologies based on microfluid-based digital PCR,such as droplet printing technology,multi-layer microfluidic chip technology,micro-bead technology,polydimethylsiloxane(PDMS)technology and so on,are discussed in detail.And the applications of microfluid-based digital PCR in quantitative detection,precise molecular diagnosis,personalized diagnosis of tumor and food safety detection are introduced.Finally,the current shortcomings and problems of microfluid-based digital PCR technologies were described,and then the future research direction and development trend of microfluid-based digital PCR technologies are prospected.

关 键 词：数字聚合酶链式反应(PCR) 微流控技术 绝对定量检测 分子诊断 食品安全 

分 类 号：Q819[生物学—生物工程] R446[医药卫生—诊断学]