LncRNAMALAT1对急性髓系白血病U937细胞增殖的影响  

Effects of LncRNA MALAT1 on the Proliferation of Acute Myeloid Leukemia U937 Cells

作　　者：王婷婷 吴燕珍 吴梅姐 王玉芳 黄楠 徐焰平 曾燕坤 WANG Tingting;WU Yanzhen;WU Meijie;WANG Yufang;Huang Nan;XU Yanping;ZENG Yankun(Quanzhou Medical College,Quanzhou,Fujian 362000,China)

机构地区：[1]泉州医学高等专科学校,福建泉州362000

出　　处：《生物化工》2023年第2期11-15,共5页Biological Chemical Engineering

基　　金：泉州医学高等专科学校校级青年科技项目(XJK1815B)。

摘　　要：目的:探讨LncRNA MALAT1对急性髓系白血病U937细胞增殖的影响。方法:急性髓细胞白血病(AML)细胞中肺腺癌转录本1(MALAT1)的表达情况采用qRT-PCR检测。转染U937细胞沉默肺腺癌转录本1(基于慢病毒载体展开),通过qRT-PCR检测干扰效果,实验组为shR-1、shR-2,对照组为shR-NC。通过CCK8法检测U937细胞增殖能力所受干扰MALAT1表达的影响。细胞凋亡相关蛋白Caspase-3的表达变化采用Western Blot方法检测。结果:AML细胞内的MALAT1表达明显提升,差异有统计学意义(P<0.01);下调MALAT1表达能明显削弱U937细胞增殖(P<0.01),提高Caspase-3表达水平。结论:U937细胞内,LncRNA MALAT1表达水平高,在U937细胞中敲降MALAT1可上调凋亡相关蛋白Caspase-3,显著抑制细胞增殖。Objective:To investigate the effect of LncRNA MALAT1 on the proliferation of acute myeloid leukemia U937 cells.Methods:The expression of MALAT1 in AML cells is detected using qRT PCR.Transfected U937 cells silenced lung adenocarcinoma transcript 1(based on Lentivirus vector),and the interference effect is detected by qRTPCR.The experimental group is shR-1,shR-2,and the control group is shR-NC.Detect the effect of interference on MALAT1 expression in U937 cell proliferation ability through CCK8 method.The expression changes of apoptosis related protein Caspase-3 are detected using Western Blot method.Results:The expression of MALAT1 in AML cells is significantly increased,with a statistically significant difference(P<0.01);downregulating the expression of MALAT1 can significantly weaken the proliferation of U937 cells(P<0.01)and increase the expression level of Caspase-3.Conclusion:In U937 cells,the expression level of LncRNA MALAT1 is high.Knocking down MALAT1 in U937 cells can upregulate the apoptosis related protein Caspase-3,significantly inhibiting cell proliferation.

关 键 词：白血病 细胞增殖 长链非编码RNA RNA干扰 

分 类 号：R733[医药卫生—肿瘤]