非肌层浸润性膀胱癌TURBT治疗患者血清UBC1和DJ-1表达水平及其对预后预测价值研究  被引量:5

Study on Serum UBC1,DJ-1 Expression Levels and Their Prognostic Value in Patients with Non Muscular Invasive Bladder Cancer Treated with TURBT

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作  者:邢俊杰 盛永亮 张浩然 刘春辉 李志军[1] XING Jun-jie;SHENG Yong-liang;ZHANG Hao-ran;LIU Chun-hui;LI Zhi-jun(Department II of Urology,the First Affiliated Hospital of Henan University of Science and Technology,Henan Luoyang 471000,China)

机构地区:[1]河南科技大学第一附属医院泌尿外二科,河南洛阳471000

出  处:《现代检验医学杂志》2023年第3期29-34,共6页Journal of Modern Laboratory Medicine

基  金:河南省医学科技攻关计划项目(LHGJ20201267)。

摘  要:目的研究非肌层浸润性膀胱癌(non-muscle invasive bladder cancer,NMIBC)患者血清长链非编码RNA尿路上皮癌相关基因1[(LncRNA)upregulated in bladder cancer 1,UBC1]、帕金森病相关蛋白-1(DJ-1)的表达情况,分析两者对NMIBC患者预后的影响。方法选择2018年2月~2019年2月于河南科技大学第一附属医院接受经尿道膀胱肿瘤电切(TURBT)治疗的120例NMIBC患者为研究对象(NMIBC组),以同期健康体检的60例健康人群为对照组。应用实时荧光定量PCR检测各组血清UBC1水平。应用酶联免疫吸附实验检测各组血清DJ-1水平。比较不同临床病理特征NMIBC患者血清UBC1和DJ-1表达差异。Kaplan-Meier生存曲线分析血清UBC1和DJ-1表达对NMIBC患者无进展生存预后的影响。单因素及多因素COX回归分析影响NMIBC患者无进展生存预后的因素。结果相比于对照组,NMIBC组血清UBC1(4.19±0.48 vs 1.27±0.29)和DJ-1(8.62±3.60 ng/ml vs 4.31±1.07 ng/ml)水平升高,差异具有统计学意义(t=43.300,12.117,均P<0.05)。肿瘤T1期、高级别NMIBC患者血清中UBC1(5.21±0.56,5.11±0.53),DJ-1(11.28±3.98 ng/ml,10.50±3.87 ng/ml)表达分别高于Ta/Tis期、低级别患者(3.79±0.43,3.64±0.44;7.34±3.04ng/ml,7.49±3.23 ng/ml),差异具有统计学意义(t=15.314,5.966;16.393,4.584,均P<0.05)。UBC1高表达组和低表达组患者的平均无进展生存时间分别为28.17±3.68个月和33.59±3.32个月。UBC1高表达组患者累积无进展生存时间低于UBC1低表达组患者,差异具有统计学意义(Log-Rank testχ^(2)=6.681,P<0.05)。DJ-1高表达组和低表达组平均无进展生存时间分别为27.34±3.29个月和34.27±3.54个月。DJ-1高表达组患者累积无进展生存时间低于DJ-1低表达组患者,差异具有统计学意义(Log-Rank testχ^(2)=11.262,P<0.05)。肿瘤分期T1期(HR=1.613,95%CI=1.223~2.126)、肿瘤分级高级别(HR=1.917,95%CI=1.314~2.799),UBC1高表达(HR=1.937,95%CI=1.229~2.745)和DJ-1高表达(HR=1.738,95%CI=1.246~2.426)是影响NObjective To study the expression of long-chain non coding RNA upregulated in bladder cancer 1(UBC1)and Parkinson’s disease associated protein-1(DJ-1)in serum of patients with non-muscle invasive bladder cancer(NMIBC),and to analyze their effects on the prognosis of patients with NMIBC.Methods 120 NMIBC patients who received TURBT treatment in the First Affiliated Hospital of He’nan University of Science and Technology from February 2018 to February 2019 were selected as the NMIBC group,and 60 healthy people who received physical examination at the same time were selected as the control group.Serum UBC1 levels were detected by real-time fluorescence quantitative PCR.The serum DJ-1 level was detected by enzyme-linked immunosorbent assay.The difference of serum UBC1 and DJ-1 expression in NMIBC patients with different clinical and pathological characteristics were compared.Kaplan Meier survival analysis were used to analyze the influence of serum UBC1 and DJ-1 level on the prognosis of progression free survival in NMIBC patients.Univariate and multivariate COX regression analysis were used to analyze factors influencing the prognosis of progression free survival in NMIBC patients.Results Compared with the control group,the serum UBC1 in NMIBC group was significantly higher(1.27±0.29 vs 4.19±0.48),the serum DJ-1 was significantly higher(4.31±1.07 ng/ml vs 8.62±3.60 ng/ml),and the differences were statistically significant(t=43.300,12.117,all P=0.000).The expression of UBC1(5.21±0.56,5.11±0.53)and DJ-1(11.28±3.98 ng/ml,10.50±3.87 ng/ml)in the serum of patients with tumor T1 and high-grade NMIBC was higher than that of patients with tumor T1 and high-grade NMIBC(3.79±0.43,3.64±0.44;7.34±3.04ng/ml,7.49±3.23 ng/ml),and the differences were statistically significant(t=15.314,5.966;16.393,4.584,all P<0.05).The mean progression free survival time of patients with high and low expression of UBC1 was 28.17±3.68 months and 33.59±3.32 months,respectively.Kaplan Meier survival analysis showed that the cumulati

关 键 词:非肌层浸润性膀胱癌 经尿道膀胱肿瘤电切术 长链非编码RNA-尿路上皮癌相关基因1 帕金森病相关蛋白-1 

分 类 号:R737.14[医药卫生—肿瘤] R730.43[医药卫生—临床医学]

 

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