长链非编码RNA CDKN2BAS在子宫内膜癌组织表达及其生物学功能研究  

Expression and Biological Function of Long Non-coding RNA CDKN2BAS in Endometrial Carcinoma

作　　者：彭浩[1] 张印星[1] 谢环[2] PENG Hao;ZHANG Yin-xing;XIE Huan(Department of Obstetrics and Gynecology,Huangshi Central Hospital/Affiliated Hospital of Hubei Polytechnic University,Hubei Huangshi 435000,China;Department of Obstetrics and Gynecology,Yichang Second People’s Hospital/the Second People’s Hospital of Three Gorges University,Hubei Yichang 443000,China)

机构地区：[1]黄石市中心医院/湖北理工学院附属医院妇产科,湖北黄石435000 [2]宜昌市第二人民医院/三峡大学第二人民医院妇产科,湖北宜昌443000

出　　处：《现代检验医学杂志》2023年第3期92-96,共5页Journal of Modern Laboratory Medicine

基　　金：湖北省卫生厅科研基金(WJ2019H524):高危型HPV的整合状态对宫颈癌细胞TRAIL凋亡的影响;2020年度湖北陈孝平科技发展基金面上项目:利用差速贴壁法分离成年小鼠卵巢生殖干细胞。

摘　　要：目的探讨长链非编码RNA CDKN2BAS(long non-coding RNA CDKN2BAS,LncRNA CDKN2BAS)在子宫内膜癌(endometrial cancer,EC)组织表达,以及沉默该基因对人EC细胞HEC-1B生物学功能的影响。方法收集2018年3月~2021年3月在黄石市中心医院接受手术治疗的107例EC患者资料,实时荧光定量聚合酶链反应(qRTPCR)检测癌组织和癌旁组织中LncRNA CDKN2BAS表达。培养HEC-1B细胞,分成siRNA组、si-NC组和C组,分别转染LncRNA CDKN2BAS抑制序列、对照序列和不作任何处理,并分别采用qRT-PCR,MTT实验和Transwell实验检测细胞中LncRNA CDKN2BAS表达及其对HEC-1B细胞增殖活性、迁移和侵袭力影响。结果EC组织中LncRNA CDKN2BAS表达量为2.73±0.25,高于癌旁组织中的1.00±0.13,差异具有统计学意义(t=63.903,P<0.001);LncRNA CDKN2BAS在FIGO分期Ⅲ~Ⅳ期(2.83±0.25)、组织学分级G3级(2.85±0.24)和出现淋巴结转移(2.84±0.26)的EC组织中的表达量较Ⅰ~Ⅱ期(2.67±0.23),G1~G2级(2.67±0.22)和未出现淋巴结转移(2.68±0.22)的EC组织明显升高(t=3.246,3.894,3.270,均P<0.05);siRNA组细胞中LncRNA CDKN2BAS表达量低于si-NC组和C组(0.30±0.12 vs 1.02±0.10,1.01±0.12),差异具有统计学意义(F=77.210,P<0.05);siRNA组细胞24,48,72和96h时A值低于si-NC组和C组,差异具有统计学意义(F=5.003~8.495,均P<0.05);siRNA组迁移细胞数和侵袭细胞数低于si-NC组和C组,差异具有统计学意义(F=21.956,22.407,均P<0.05)。结论EC组织中LncRNA CDKN2BAS表达量升高,沉默人EC细胞HEC-1B中LncRNA CDKN2BAS表达可抑制细胞增殖活性,减少细胞迁移和侵袭数量。Objective To explore the expression of long non-coding RNA CDKN2BAS(LncRNA CDKN2BAS)in endometrial cancer(EC)tissues,and the effect of silencing this gene on the biological functions of human EC cells HEC-1B.Methods The data of 107 cases of EC patients who underwent surgical treatment in Huangshi Central Hospital were collected from March 2018 to March 2021,and qRT-PCR was used to detect the expression of LncRNA CDKN2BAS in the cancer tissues and adjacent tissues.HEC-1B cells was cultured and divided into siRNA group,si-NC group and C group,and transfected with LncRNA CDKN2BAS inhibitory sequence,control sequence and without any treatment,respectively.The qRT-PCR,MTT experiment and Transwell experiment were used to detect the expression of LncRNA CDKN2BAS in cells and its effect on the cell proliferation activity,migration and invasion of HEC-1B cells.Results The expression level of LncRNA CDKN2BAS in EC tissues was 2.73±0.25,which was higher than 1.00±0.13 in the adjacent tissues,the difference was statistically significant(t=63.903,P<0.001).The expression levels of LncRNA CDKN2BAS in EC tissues with FIGO stage III~IV(2.83±0.25),histological grade G3(2.85±0.24)and lymph node metastasis(2.84±0.26)were significantly higher than those in EC tissues with stage I~II(2.67±0.23),G1~G2(2.67±0.22)and no lymph node metastasis(2.68±0.22)(t=3.246~3.894,all P<0.05).The expression level of LncRNA CDKN2BAS in the cells in the siRNA group was lower than that in the si-NC group and the C group(0.30±0.12 vs 1.02±0.10,1.01±0.12),the difference was statistically significant(F=77.210,P<0.05).And the A values of cells at 24,48,72 and 96h in the siRNA group were lower than those in the si-NC group and the C group,the differences were statistically significant(F=5.003~8.495,all P<0.05).The number of migrating cells and the number of invasive cells in the siRNA group were lower than those in the si-NC group and the C group,the differences were statistically significant(F=21.956,22.407,all P<0.05).Conclusion The expression lev

关 键 词：子宫内膜癌 长链非编码RNA-CDKN2BAS 细胞增殖 细胞侵袭 

分 类 号：R737.33[医药卫生—肿瘤] R730.43[医药卫生—临床医学]