溃疡性结肠炎患者血清LncRNA Mirt2和LncRNA IFNG-AS1表达水平及与预后相关性研究  

Study on the Expression Levels of Serum LncRNA Mirt2 and LncRNA IFNGAS1 in Patients with Ulcerative Colitis and Their Correlation with Prognosis

作　　者：王飞[1] 祝靳 赵宝林[1] WANG Fei;ZHU Jin;ZHAO Bao-lin(Department of Anorectal,Nanjing Pukou District Traditional Chinese Medicine Hospital/Nanjing Traditional Chinese Medicine Hospital Pukou Branch,Nanjing 211800,China)

机构地区：[1]南京市浦口区中医院/南京市中医院浦口分院肛肠科,南京211800

出　　处：《现代检验医学杂志》2023年第3期114-118,148,共6页Journal of Modern Laboratory Medicine

基　　金：南京市浦口区社会事业科技发展计划项目(S2021-22)。

摘　　要：目的探讨长链非编码RNA心肌梗死相关转录本2(long non-coding RNA myocardial infarction-related transcript 2,LncRNA Mirt2)、长链非编码RNA干扰素γ-反义RNA1(long non-coding RNA interferonγ-antisense RNA 1,LncRNA IFNG-AS1)在溃疡性结肠炎(ulcerative colitis,UC)患者血清中表达情况及与UC患者预后相关性。方法选择2018年12月~2021年1月南京市浦口区中医院(南京市中医院浦口分院)收治的193例UC患者作为观察对象,其中缓解期85例,活动期108例。根据患者预后情况分为复发组(n=78)和未复发组(n=115),另选取同期190例健康体检者作为对照组。采用实时荧光定量PCR(quantitative real-time PCR,qRT-PCR)检测血清LncRNA Mirt2和LncRNA IFNG-AS1表达水平,Pearson法分析UC患者血清LncRNA Mirt2和LncRNA IFNG-AS1表达水平相关性及LncRNA Mirt2和LncRNA IFNG-AS1与C-反应蛋白(C-reactive protein,CRP)、白细胞介素-6(interleukin-6)和肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)的相关性;ROC曲线分析血清LncRNA Mirt2和LncRNA IFNG-AS1对复发UC的诊断价值。结果与对照组比较,缓解期组与活动期组UC患者血清LncRNA Mirt2(0.92±0.10,0.71±0.08vs 1.07±0.12)表达水平降低,LncRNA IFNG-AS1(1.63±0.25,2.27±0.42 vs 1.01±0.14)表达水平及CRP(13.42±4.71mg/L,25.38±6.29 mg/L vs 4.32±1.27 mg/L),IL-6(8.31±2.35pg/ml,16.55±4.52 pg/mlvs 2.87±0.78 pg/ml),TNF-α(15.38±4.29 pg/ml,28.94±6.37 pg/ml vs 8.42±1.63 pg/ml)水平升高,差异具有统计学意义(t=10.064~44.632,均P<0.001);与缓解期组相比,活动期组UC患者血清LncRNA Mirt2表达水平降低,LncRNA IFNG-AS1表达水平及CRP,IL-6,TNF-α水平升高,差异具有统计学意义(t=12.420~16.844,均P<0.001);与轻度组比较,中、重度组UC患者血清LncRNA Mirt2(0.69±0.08,0.58±0.05 vs 0.81±0.06)表达水平降低,LncRNA IFNG-AS1(2.25±0.48,2.83±0.25 vs 1.90±0.31)表达水平升高,差异有统计意义(t=3.890~17.225,均P<0.001);与中度组比较,重度组UC患者血清LncRNA Mirt2表达水平�Objective To investigate the expression of long non-coding RNA myocardial infarction-related transcript 2(LncRNA Mirt2)and long non-coding RNA interferonγ-antisense RNA 1(LncRNA IFNG-AS1)in serum of patients with ulcerative colitis(UC)and their correlation with prognosis of UC patients.Methods A total of 193 UC patients admitted to Nanjing Pukou District Traditional Chinese Medicine Hospital(Nanjing Traditional Chinese Medicine Hospital Pukou Branch)from December 2018 to January 2021 were selected as observation objects including 85 cases in remission stage,108 cases in active stage.According to the prognosis of patients,they were grouped into a recurrence group(n=78)and a non-recurrence group(n=115).In addition,190 healthy subjects were taken as the control group.The expression levels of serum LncRNA Mirt2 and LncRNA IFNG-AS1 were measured by Quantitative Real-time PCR(qRT-PCR),the correlation between serum LncRNA Mirt2,LncRNA IFNG-AS1 expression levels,LncRNA Mirt2,LncRNA IFNG-AS1 and C-reactive protein,Interleukin-6(IL-6)and Tumor necrosis factor-α(TNF-α)were analyzed by Pearson method,and the diagnostic value of serum LncRNA Mirt2 and LncRNA IFNG-AS1 in recurrent UC was analyzed by ROC curve.Results Compared with the control group,the expression levels of serum LncRNA Mirt2(0.92±0.10,0.71±0.08 vs 1.07±0.12)in UC patients in the remission and active groups were decreased,LncRNA IFNG-AS1(1.63±0.25,2.27±0.42 vs 1.01±0.14)and CRP(13.42±4.71mg/L,25.38±6.29 mg/L vs 4.32±1.27 mg/L),IL-6(8.31±2.35pg/ml,16.55±4.52 pg/ml vs 2.87±0.78 pg/ml)and TNF-α(15.38±4.29 pg/ml,28.94±6.37 pg/ml vs 8.42±1.63 pg/ml)levels increased,and the differences were statistically significant(t=10.064~44.632,all P<0.001).Compared with the remission group,the serum LncRNA Mirt2 expression level of UC patients in the active group was decreased(0.71±0.08 vs 0.92±0.10),LncRNA IFNG-AS1(2.27±0.42 vs 1.63±0.25)expression level and CRP,IL-6 and TNF-αlevels increased,and the differences were statistically significant(t=12.420~16

关 键 词：溃疡性结肠炎 长链非编码RNA心肌梗死相关转录本2 长链非编码RNA干扰素γ-反义RNA1 

分 类 号：R574.62[医药卫生—消化系统] R392.11[医药卫生—内科学]