乳腺生物反应器的表达优化策略  

Strategies to Optimize the Expression of Mammary Gland Bioreactor

作　　者：刘晓燕 祝振亮 史广宇 华梓宇 杨晨 张涌[1] 刘军[1] LIU Xiao-yan;ZHU Zhen-liang;SHI Guang-yu;HUA Zi-yu;YANG Chen;ZHANG Yong;LIU Jun(College of Veterinary Medicine,Northwest A&F University,Yangling 712100)

机构地区：[1]西北农林科技大学动物医学院,杨凌712100

出　　处：《生物技术通报》2023年第5期77-91,共15页Biotechnology Bulletin

基　　金：国家自然科学基金面上项目(32072805)。

摘　　要：乳腺生物反应器是指将外源基因导入动物基因组并在动物乳腺中特异性表达,利用动物乳腺合成、分泌蛋白的功能,在其乳汁中获得外源蛋白的技术。乳腺生物反应器凭借其高表达、低成本以及合成蛋白质的结构接近天然蛋白质等优势而被视为药用和营养蛋白生产的一次技术革新,然而由于外源基因随机整合以及重组蛋白表达不稳定等问题极大地限制了其应用。本文结合乳腺生物反应器的发展现状,从利用基因编辑技术、筛选合适的外源基因整合位点以及改进外源基因调控序列3个方面对乳腺生物反应器优化策略进行了综述,以期为提高乳腺生物反应器生产重组蛋白的表达提供理论借鉴。Mammary gland bioreactor refers to the technology of importing foreign genes into animal genomes which express specifically in animal mammary gland,and using the function of synthesizing and secreting proteins of mammary glands to obtain recombinant proteins in the milk.Mammary gland bioreactor is regarded as a technological innovation for the production of medicinal protein and nutritional protein in the bio-pharmaceutical industry due to its advantages of high expression,low cost and synthesized protein being close to natural proteins in structure.However,due to the random integration of foreign genes and unstable expression of recombinant proteins,its application is largely limited.Based on the current development status of mammary gland bioreactor,this paper reviewed the optimization strategies of mammary gland bioreactor from three perspectives:using gene editing technology,screening suitable foreign gene integration sites and improving the regulatory sequence of exogenous proteins,so as to provide theoretical reference for improving the expression of recombinant protein produced by mammary gland bioreactor.

关 键 词：乳腺生物反应器 染色质开放性 定向整合 CRISPR/Cas9 调控序列 打靶位点 

分 类 号：R73[医药卫生—肿瘤]