SP1调控碳水化合物分配对穗形态的影响  

Effect of SP1 on Panicle Architecture by Regulating Carbohydrate Remobilization

作　　者：梁成刚 汪燕 李天[3] 大杉立[2] 青木直大 LIANG Cheng-gang;WANG Yan;LI Tian;OHSUGI Ryu;AOKI Naohiro(Institute of Plant Genetics and Breeding,College of Life Sciences,Guizhou Normal University,Guiyang 550001;Faculty of Agriculture,The University of Tokyo,Tokyo 113-8657,Japan;College of Agronomy,Sichuan Agricultural University,Chengdu 611130)

机构地区：[1]贵州师范大学生命科学学院植物遗传育种研究所,贵阳550001 [2]日本东京大学农学部,日本东京113-8657 [3]四川农业大学农学院,成都611130

出　　处：《生物技术通报》2023年第5期152-159,共8页Biotechnology Bulletin

基　　金：国家留学基金委员会项目(201206910010);贵州省高层次创新型千层次人才项目(111/0920008);贵州师范大学学术新苗基金项目(黔师新苗[2021]A17号)。

摘　　要：探讨SP1在水稻“源-库”系统中的调控作用,为解析稻穗形成的机理和青贮饲料稻分子育种提供科学参考。以sp1与WT为材料,进行氮处理和生长分析,表型鉴定、碳水化合物测定和RT-qPCR检测。结果发现,氮处理10 d时,sp1与WT无表型差异,但在高、中氮条件下,sp1相对生长速率显著提高;24 d时sp1叶鞘、根、植株干重提高,叶鞘氮含量降低、碳/氮比值提高,说明充裕的氮能促进sp1生长,SP1可能影响叶鞘碳-氮平衡。抽穗期sp1株高显著降低,分蘖数显著提高,稻穗变小并伴随高位分蘖发生。孕穗期sp1茎、鞘中碳水化合物含量与WT差异较小,但抽穗期茎中淀粉、蔗糖和叶鞘中淀粉、蔗糖、葡萄糖和果糖含量明显提高。SP1在叶鞘基部高表达,推测突变SP1能抑制蔗糖由茎鞘向穗的运输,导致高位分蘖发生和稻穗变小。SP1可应用于青贮饲料稻分子育种。In order to explore the regulation of SP1 in the rice“source-sink”system and provide scientific reference for understanding the mechanism of rice panicle formation and molecular breeding of whole-rice silage,sp1 and WT were selected as materials for nitrogen treatment and plant growth analysis,phenotype identification,carbohydrate determination,and RT-qPCR detection.The results indicated that there was no phenotypic difference between sp1 and WT at 10 d after nitrogen treatment.However,sp1 showed significantly higher plant relative growth rate than WT under high and medium nitrogen treatment.Meanwhile,sp1 demonstrated higher value of the dry weight of leaf sheath,root and plant than WT at 24 d after nitrogen treatment,while a decrease in the content of nitrogen and an increase in the value of C/N ratio in leaf sheath.It was speculated that the abundant nitrogen improved plant growth of sp1,but the nitrogen stress impaired carbon-nitrogen balance in the leaf sheath.At heading stage,sp1 showed a significant decrease in plant height and a significant increase in tiller number,and shorter panicle with the occurrence of high-node tillering.At booting stage,there was little difference of the carbohydrates content in the stem and sheath between sp1 and WT.But,sp1 showed higher content of starch,sucrose in stem,and higher content of starch,sucrose,glucose and fructose in the leaf sheath at heading stage.SP1 highly expressed at the base of leaf sheath,which implied that the mutation of SP1 would inhibit the sucrose transport from the stem and sheath to the panicle and induce high-node tillering and short panicle.Rice SP1 gene is a candidate gene for molecular breeding of whole-rice silage.

关 键 词：穗形态 SP1 碳水化合物 叶鞘 青贮饲料稻 

分 类 号：S51[农业科学—作物学]