植物精油和胆盐复合制剂对山羊肠道屏障功能的影响  

Effects of Plant Essential Oil and Bile Salt Compound Preparation on Intestinal Barrier Function of Goats

作　　者：高瑞玲 李胜利 金鹿 张春华 张崇志 章嘉诚 孙海洲 GAO Ruiling;LI Shengli;JIN Lu;ZHANG Chunhua;ZHANG Chongzhi;ZHANG Jiacheng;SUN Haizhou(Inner Mongolia Key Laboratory of Herbivores Nutrition,Animal Nutrition and Feed Research Institution,Inner Mongolia Academy of Agriculture and Animal Husbandry,Hohhot 010031,China)

机构地区：[1]内蒙古自治区农牧业科学院动物营养与饲料研究所,内蒙古自治区草食动物营养科学重点实验室,呼和浩特010031

出　　处：《动物营养学报》2023年第5期3174-3182,共9页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家绒毛用羊产业技术体系(CARS-39-11);国家重点研发计划(2022YFD1301102);国家自然科学基金(M2142006);“科技兴蒙”行动重点专项项目(KJXM-EEDS-2020002);内蒙古农牧业创新基金(2021CXJJM03);鄂尔多斯市科技重大专项项目(2022EEDSKJZDZX024);农业农村部草食家畜健康养殖与畜产品品质调控重点实验室项目;内蒙古自治区草食动物营养科学重点实验室项目。

摘　　要：本试验旨在研究饲粮中添加植物精油和胆盐复合制剂对山羊肠道屏障功能的影响。选择24只5月龄的体重为(21.46±2.07)kg的阿拉善白绒山羊公羊,随机分为4组,每组6个重复,每个重复1只。对照组饲喂基础饲粮,试验组均在基础饲粮中额外添加5 g/kg以麦饭石为载体的复合制剂;其中试验1组(T1组)复合制剂中含500 mg植物精油(60%沙蒿油、40%香芹酚+肉桂醛+牛至油),试验2组(T2组)复合制剂中含500 mg植物精油(60%沙蒿油、40%香芹酚+肉桂醛+牛至油)和500 mg胆盐,试验3组(T3组)复合制剂中含500 mg胆盐。预试期10 d,正试期60 d。结果表明:1)与对照组相比,T1组T2组的平均日采食量显著提高(P<0.05);T1组的料重比降低了28%,T2组的料重比降低了10%。2)与对照组相比,T1、T2组的空肠绒毛高度、绒毛高度/隐窝深度显著升高(P<0.05),T1组的空肠隐窝深度显著降低(P<0.05);T1组的回肠绒毛高度和绒毛高度/隐窝深度显著升高(P<0.05),T1、T2组的回肠隐窝深度显著降低(P<0.05)。3)与对照组相比,T1组的盲肠黏膜闭锁小带蛋白-1(ZO-1)的mRNA相对表达量显著提高(P<0.05);T2和T3组的瘤胃闭锁蛋白(Occludin)的mRNA相对表达量显著提高(P<0.05),T1和T2组的回肠黏膜Occludin的mRNA相对表达量显著提高(P<0.05);T1和T3组的盲肠黏膜封闭蛋白-1(Claudin-1)的mRNA相对表达量显著提高(P<0.05);T1、T2和T3组的肝脏法尼醇X受体(FXR)的mRNA相对表达量显著提高(P<0.05)。由此可见,饲粮中添加复合植物精油对山羊平均日采食量、料重比以及肠道组织形态发育效果最佳,对肠道紧密连接蛋白的mRNA表达有促进作用;饲粮中添加植物精油和胆盐复合制剂在一定程度上也能提高山羊平均日采食量、降低料重比,且在肠道健康发育过程中也有积极影响。This experiment was conducted to investigate the effects of plant essential oil and bile salt compound preparation on intestinal barrier function of goats.Twenty-four 5-month-old Alxa white cashmere goat rams with body weight of(21.46±2.07)kg were randomly divided into 4 groups with 6 replicates in each group and 1 goat in each replicate.The control group was fed a basal diet,and the experimental groups were fed basal diet supplemental with 5 g/kg compound preparation with medical stone as carrier;among them,the compound preparation in experimental group 1(T1 group)contained 500 mg plant essential oil(60%artemisia oil,40%carvacrol+cinnamaldehyde+oregano oil),the compound preparation in experimental group 2(T2 group)contained 500 mg plant essential oil(60%artemisia oil,40%carvacrol+cinnamaldehyde+oregano oil)and 500 mg bile salt,and the compound preparation in experimental group 3(T3 group)contained 500 mg bile salt.The pre-feeding period was 10 days and the feeding period was 60 days.The results showed as follows:1)compared with the control group,the average daily feed intake of T1 group and T2 group was significantly increased(P<0.05);the feed to gain ratio of T1 group was decreased by 28%,and the feed to gain ratio of T2 group was decreased by 10%.2)Compared with the control group,the villous height and villous height/crypt depth in jejunum of T1 and T2 groups were significantly increased(P<0.05),and the crypt depth in jejunum of T1 group was significantly decreased(P<0.05);the villous height and villous height/crypt depth in ileum of T1 group were significantly increased(P<0.05),and the crypt depth in ileum of T1 and T2 groups was significantly decreased(P<0.05).3)Compared with the control group,the mRNA relative expression level of zonula occluden-1(ZO-1)in cecal mucosa of T1 group was significantly increased(P<0.05);the mRNA relative expression level of Occludin in rumen of T2 and T3 groups was significantly increased(P<0.05),and the mRNA relative expression level of Occludin in ileal mucosa of T1 and T2 gr

关 键 词：幼龄绒山羊 植物精油 胆盐 肠道屏障 

分 类 号：S827[农业科学—畜牧学]