脯氨酸对中华鳖生长性能、抗氧化能力和抗嗜水气单胞菌感染能力的影响  

Effects of Proline on Growth Performance,Antioxidant Capacity and Resistance to Aeromonas hydrophila Infection of Pelodiscus sinensis

作　　者：邓紫连 宋伟[1] 芮千龙 石菖昊 张骥凯 李彩燕[1] DENG Zilian;SONG Wei;RUI Qianlong;SHI Changhao;ZHANG Jikai;LI Caiyan(College of Biology and Environment,Zhejiang Wanli University,Ningbo 315000,China)

机构地区：[1]浙江万里学院生物与环境学院,宁波315000

出　　处：《动物营养学报》2023年第5期3213-3227,共15页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：浙江省农业重大技术协同推广计划项目(2022XTTGSC05);浙江省生物工程一流学科创新项目(CX2021022);宁波市科技创新2025重大专项(2021Z009);宁波市自然科学基金重点项目(2022J186)。

摘　　要：本研究旨在探讨脯氨酸对中华鳖生长性能、抗氧化能力和抗嗜水气单胞菌感染能力的影响。试验选取初始体重为200 g左右的健康中华鳖幼鳖48只,随机分为对照组(P0组)和脯氨酸组(P4组),分别饲喂添加0(基础饲料)和40 g/kg脯氨酸的饲料,每组3个重复,每个重复8只。试验期为42 d。养殖试验结束后,从各组随机选择3只中华鳖经腹腔注射1 mL的嗜水气单胞菌(Ah)菌液,分别标记为P0+Ah组和P4+Ah组;再各组随机选择另3只中华鳖经腹腔注射等量无菌生理盐水,分别标记为P0+NS组和P4+NS组。攻毒24 h后,取样测定相关指标。结果表明:1)与对照组相比,脯氨酸组中华鳖存活率、增重率和特定生长率显著提高(P<0.05),但肝体比和脏体比无显著差异(P>0.05)。2)与P0+NS组相比,P4+NS组中华鳖裙边组织中谷胱甘肽过氧化物酶(GSH-Px)和超氧化物歧化酶(SOD)活性显著提高(P<0.05),且线粒体活性氧(ROS)产生速率显著降低(P<0.05)。在Ah应激作用下,与P0+Ah组相比,P4+Ah组裙边组织中GSH-Px和SOD活性显著提高(P<0.05),而线粒体ROS产生速率显著降低(P<0.05)。3)与P0+NS组相比,P4+NS组中华鳖裙边组织中脯氨酸和羟脯氨酸含量显著提高(P<0.05)。在Ah应激作用下,与P0+Ah组相比,P4+Ah组裙边组织中脯氨酸和羟脯氨酸含量显著提高(P<0.05)。4)与P0+NS组相比,P4+NS组中华鳖裙边组织中Ⅰ型胶原蛋白A1(COL1A1)、Ⅰ型胶原蛋白A2(COL1A2)、脯氨酰4-羟化酶A1(P4HA1)、脯氨酰4-羟化酶A3(P4HA3)、转化生长因子-β2(TGF-β2)和转化生长因子-β3(TGF-β3)mRNA相对表达量显著提高(P<0.05),组织金属蛋白酶抑制因子2(TIMP2)和组织金属蛋白酶抑制因子4(TIMP4)mRNA相对表达量显著降低(P<0.05)。在Ah应激作用下,与P0+Ah组相比,P4+Ah组裙边组织中COL1A1、COL1A2、P4HA1、P4HA3、TGF-β2、TGF-β3、基质金属蛋白酶1(MMP1)、基质金属蛋白酶2(MMP2)、TIMP2和TIMP4 mRNA相对表达量均显著提高(P<0.05)。5)组织The aim of this study was to investigate the effects of proline on growth performance,antioxidant capacity and resistance to Aeromonas hydrophila infection of Pelodiscus sinensis.Forty-eight healthy young Pelodiscus sinensis with an initial body weight of about 200 g were randomly divided into control group(P0 group)and proline group(P4 group),which fed diets supplemented with 0(basal diet)and 40 g/kg proline,respectively,and each group had 3 replicates and each replicate had 8 turtles.The experiment lasted for 42 days.After the feeding experiment,3 turtles were randomly selected from each group to be intraperitoneally injected with 1 mL Aeromonas hydrophila(Ah)bacteria solution,and labeled as P0+Ah group and P4+Ah group,respectively;another 3 turtles were randomly selected in each group to be intraperitoneally injected with the same amount of sterile normal saline and labeled as P0+NS group and P4+NS group,respectively.After 24 h of challenge,the relevant indices were determined by sampling.The results showed as follows:1)compared with the control group,the survival rate,weight gain rate and specific growth rate of Pelodiscus sinensis in proline group were significantly increased(P<0.05),but there were no significant differences in hepatosomatic ratio and viscerosomatic ratio(P>0.05).2)Compared with P0+NS group,the activities of glutathione peroxidase(GSH-Px)and superoxide dismutase(SOD)in calipash tissues of Pelodiscus sinensis in P4+NS group were significantly improved(P<0.05),and the production rate of mitochondrial reactive oxygen species(ROS)was significantly decreased(P<0.05).Under Ah stress,compared with P0+Ah group,the activities of GSH-Px and SOD in calipash tissues in P4+Ah group were significantly increased(P<0.05),while the production rate of mitochondrial ROS was significantly decreased(P<0.05).3)Compared with P0+NS group,the content of proline and hydroxyproline in calipash tissues of Pelodiscus sinensis in P4+NS group was significantly improved(P<0.05).Under Ah stress,compared with P0+Ah group,the

关 键 词：脯氨酸 嗜水气单胞菌 抗氧化能力 胶原蛋白 中华鳖 

分 类 号：S963[农业科学—水产养殖]