真菌纤溶化合物1的遗传毒性评价  

Evaluation of genotoxicity of fungal fibrinolytic compound 1

作　　者：侍雯婧 田逸君 董雅春 吴文惠[2] 张添 张惠姝 朱玉平 SHI Wenjing;TIAN Yijun;DONG Yachun;WU Wenhui;ZHANG Tian;ZHANG Huishu;ZHU Yuping(Department of Hygeine and Toxicology,Faculty of Naval Medicine,Naval Medical University,Shanghai 200433;Centre for Marine Medicine and Health Food Research,School of Food Science and Technology,Shanghai Ocean University,Shanghai 201306;Experimental Teaching Center of Basic Medical College,Naval Medical University,Shanghai 200433,China)

机构地区：[1]海军军医大学海军医学系卫生毒理学教研室,上海200433 [2]上海海洋大学食品学院海洋药物与健康食品研究中心,上海201306 [3]海军军医大学基础医学院实验教学中心,上海200433

出　　处：《癌变．畸变．突变》2023年第3期219-223,共5页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金(82173731);上海市自然科学基金(21ZR1427300)。

摘　　要：目的:在已建立的良好实验室规范(GLP)平台,对真菌纤溶化合物1(FGFC1)进行系统的遗传毒性评估。方法:采用经典遗传毒性检测组合(Ames试验、体外培养CHO细胞染色体畸变试验和小鼠骨髓细胞微核试验)检测FGFC1的遗传毒性。结果:Ames试验结果提示,FGFC1在每皿0.5、5、50、500、1000 mg 5个剂量下,在加和不加代谢活化系统(S9)时,对鼠伤寒沙门氏菌均无致突变性。CHO细胞染色体畸变试验结果提示,在终浓度62.5、125.0和250.0 mg/mL 3个剂量组,在加和不加S9时,于作用24 h和48 h的条件下培养的CHO细胞,均未诱发染色体畸变。小鼠骨髓细胞微核试验在62.5、125.0和250.0 mg/kg 3个剂量下对骨髓细胞的微核诱发率,与溶剂对照组比较差异均无统计学意义(P>0.05)。结论:在本实验条件下,未检测到FGFC1的遗传毒性和潜在致癌性。OBJECTIVE:To perform a systematic genotoxicity assessment of fungal fibrinolytic compound 1(FGFC1)in an established GLP laboratory platform.METHODS:A combination of classical genotoxicity assays(Ames,in vitro culture CHO cell chromosome aberration and mouse bone marrow micronucleus assays)was used to detect genotoxicity of FGFC1.RESULTS:Results from the Ames assay indicate that FGFC1 was not mutagenic to Salmonella typhimurium at five doses of 0.5,5,50,500 and 1000 g per dish,with and without the addition of the S9 metabolic activation system.FGFC1 did not induce chromosomal aberrations in the presence of S9 after 24 h and 48 h of treatments.The rates of micronucleus induction in bone marrow cells at 3 doses of 62.5,125.0 and 250.0 mg/kg was not significantly different from that in the solvent control group(P>0.05).CONCLUSION:The above results indicate that FGFC1 was not genotoxic and not potentially carcinogenic under the test conditions.

关 键 词：真菌纤溶化合物 AMES试验 染色体畸变试验 微核实验 遗传毒性 

分 类 号：R394.6[医药卫生—医学遗传学]