芹菜种子3次醇层物对黄瓜枯萎病菌的抑菌活性及化学物质GC-MS初步分析  

Antifungal Activity of Celery Seed Three Times Ethanol Extracts on Fusarium oxysporum f.sp.cucumerinum and Preliminary Analysis of Chemical Substances by GC-MS

作　　者：钱程 赵雪梅 郭成 周玉雷 秦立金 云兴福[3] QIAN Cheng;ZHAO Xuemei;GUO Cheng;ZHOU Yulei;QIN Lijin;YUN Xingfu(College Agriculture,Chifeng University,Chifeng 024000,Inner Mongolia,China;College of Chemistry and Life Science,Chifeng University,Chifeng 024000,Inner Mongolia,China;College of Horticulture and Plant Protection,Inner Mongolia Agriculture University,Hohhot 010019,Inner Mongolia,China)

机构地区：[1]赤峰学院农学院,内蒙古赤峰024000 [2]赤峰学院化学与生命科学学院,内蒙古赤峰024000 [3]内蒙古农业大学园艺与植物保护学院,内蒙古呼和浩特010019

出　　处：《中国蔬菜》2023年第6期77-84,共8页China Vegetables

基　　金：内蒙古自治区自然科学基金项目(2015BS0309);内蒙古自治区高等学校科学研究项目(NJZY22191);赤峰学院一流学科建设项目(CFXYYLXKB202102);蒙东地区生物资源开发利用创新团队项目(cfxykycxtd202008)。

摘　　要：为了明确芹菜种子中含有的抑制黄瓜枯萎病菌的植物化学物质成分,以津南实芹1号种子为试材,采用乙醇浸提法获得粗提物,再进行连续3次柱层析分离纯化,对得到的流分进行黄瓜枯萎病菌皿内抑菌试验;利用气相色谱-质谱(GCMS)法对第3次层析分离获得的最佳流分进行分析,探究芹菜种子3次醇层物中抑制黄瓜枯萎病菌的植物化学物质种类及成分。结果表明:芹菜种子乙醇粗提物经3次层析分离获得的不同流分均可抑制黄瓜枯萎病菌菌丝生长和孢子形成,4个最佳流分SE655、SE667、SE767、SE777的孢子形成抑制率分别为88.30%、87.82%、84.74%、86.24%,菌丝生长抑制率分别为25.21%、36.88%、35.95%、25.81%。GC-MS分析表明,4个最佳流分所含有的抑菌植物化学物质种类丰富,含量不同;初步分离鉴定出共10类36种化合物,包含烷烃9种,酯类8种,有机酸6种,有机胺4种,酚类3种,醛类2种,醇类1种,芳香烃1种,杂环化合物1种,植物毒素1种;其中,酯类、有机胺和酚类相对含量较高。In order to explicit the phytochemical constituents inhibiting Fusarium oxysporum f.sp.cucumerinum and contained in celery seed,this study took celery seed‘Jinnanshiqin No.1’as test material;adopted ethanol extraction method to obtain crude extract;then conducted column chromatography separation and purifi cation for 3 consecutive times;carried out test in dishes for the inhibition of Fusarium oxysporum f.sp.cucumerinum on obtained fl uid fraction;analyzed the optimal fl ow fraction obtained by the third chromatography using gas chromatography-mass spectrometry(GC-MS);and studied the kinds and components of phytochemicals inhibiting Fusarium oxysporum f.sp.cucumerinum in the tertiary alcohols of celery seed.Results indicated that the different flow fractions of ethanol extract from celery seed obtained by 3 chromatographic separation could inhibit the mycelium growth and spore formation of Fusarium oxysporum f.sp.cucumerinum.The 4 optimal fractions were SE655,SE667,SE767 and SE777.Their inhibition rates for spore formation were 88.30%,87.82%,84.74%,86.24%,respectively;for mycelium growth were 25.21%,36.88%,35.95%and 25.81%,respectively.The GC-MS analysis showed that the kinds of bacteriostatic phytochemicals contained by the 4 optimal fractions were abundant with different contents.Tentatively,10 classes of 36 compounds were isolated and identified,including 9 kinds of alkanes,8 kinds of esters,6 kinds of organic acids,4 kinds of organic amines,3 kinds of phenolic compounds,2 kinds of aldehydes,1 kind of alcohol,1 kind of aromatic hydrocarbon,1 kind of heterocyclic compound and 1 kind of phytotoxic.Among them,the relative amounts of esters,organic amines and phenolic compounds were higher.

关 键 词：芹菜 种子 醇层物 黄瓜枯萎病菌 GC-MS 植物化学物质 

分 类 号：S436.421.13[农业科学—农业昆虫与害虫防治]