Chemical tools for E3 ubiquitin ligase study  

作　　者：Yangwode Jing Chong Zuo Yun-Xiang Du Junxiong Mao Ruichao Ding Jiachen Zhang Lu-Jun Liang Qian Qu 

机构地区：[1]Institute of Translational Medicine,Shanghai Jiao Tong University,Shanghai 200240,China [2]Tsinghua-Peking Center for Life Sciences,Ministry of Education Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology,Center for Synthetic and Systems Biology,Department of Chemistry,Tsinghua University,Beijing 100084,China

出　　处：《Chinese Chemical Letters》2023年第4期67-74,共8页中国化学快报（英文版）

摘　　要：E3 ubiquitin ligases catalyze the final step of ubiquitylation,a crucial post-translational modification involved in almost every process in eukaryotic cells.E3 ubiquitin ligases are key regulators of cellular events,and the investigation into their functions and functioning mechanisms are research areas with great importance.Synthetic or semi-synthetic tools have greatly facilitated the research about the enzyme activity,distribution in different physiological events,and catalytic mechanism of E3 ubiquitin ligase.In this review,we summarize the development of chemical tools for E3 ubiquitin ligases with an emphasis on the synthetic routes.We show the utility of these chemical tools by briefly discussing their applications in biological research.

关 键 词：Chemical tools Protein chemical synthesis UBIQUITYLATION E3 ligase Enzymatic mechanism 

分 类 号：Q55[生物学—生物化学] TQ426.97[化学工程]