Synthesis and immunological study of a glycosylated wall teichoic acid-based vaccine against Staphylococcus aureus  

作　　者：Peng Shen Han Lin Yikai Bao Haofei Hong Zhimeng Wu 

机构地区：[1]Key Laboratory of Carbohydrate Chemistry&Biotechnology,Ministry of Education,School of Biotechnology,Jiangnan University,Wuxi 214122,China

出　　处：《Chinese Chemical Letters》2023年第4期193-197,共5页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China (Nos. 21472070 and 22177040);partly supported by the 111 Project (No. 111-2-06)。

摘　　要：Staphylococcus aureus wall teichoic acids(WTAs) are attractive targets for antibacterial vaccine development. In this study, three core glycosylated WTA structure, including α-1,4-Glc NAc, β-1,4-Glc NAc andβ-1,3-Glc NAc modified ribitol phosphates containing a linker are chemically synthesized and conjugated with tetanus toxin(TT) carrier protein as vaccine candidates. In vivo immunological studies demonstrate that the synthesized glycosylated WTAs display high immunogenicity and all conjugates provoke strong immune responses and elicit high levels of specific IgG antibodies against the Glc NAc-modified WTA. Furthermore, antibodies elicited by the vaccine candidates remain the capability to recognize S. aureus cells and display significant opsonophagocytic activity to clear S. aureus. This study demonstrates that the core structure of glycosylated WTAs are effective antigens for constructing anti-S. aureus vaccines to prevent and control S. aureus infections.

关 键 词：WTAs Staphylococcus aureus GlcNAc modifications GLYCOCONJUGATES Antibacterial vaccine 

分 类 号：TQ464[化学工程—制药化工]