Rationally designed meso-benzimidazole-pyronin with emission wavelength beyond 700 nm enabling in vivo visualization of acute-liver-injury-induced peroxynitrite  

作　　者：Minghao Ren Chengyong Zhou Linfang Wang Xin Lv Wei Guo 

机构地区：[1]Department of Chemistry,Changzhi University,Changzhi 046011,China [2]School of Chemistry and Chemical Engineering,Shanxi University,Taiyuan 030006,China

出　　处：《Chinese Chemical Letters》2023年第4期226-230,共5页中国化学快报（英文版）

基　　金：Scientific and Technological Innovation Program of Colleges and Universities in Shanxi Province (Nos.2021L529, 2021L530)。

摘　　要：Fluorescent dyes with fluorescence emission above 700 nm are favorable for bio-imaging due to the higher tissue transparency and lower background fluorescence. In this study, we present a mesobenzimidazole-pyronin platform(Si BMs) with fluorescence emission maxima above 700 nm, which possess good cell permeability, photostability, and lysosomal localization. The great photophysical properties of the Si BMs encouraged us to further exploit their application toward bio-imaging. We synthesized the reduced ‘dihydro’ derivative HSi BM3 for sensing ONOO^(-), with high selectivity and sensitivity and a fast fluorescence “off-on” response(within 2 s). Then, we confirmed the potential of HSi BM3 for visualizing exogenous and endogenous ONOO-in cells and mice. More importantly, HSi BM3 was successfully employed for visualizing acute-liver-injury-induced peroxynitrite.

关 键 词：Near infrared fluorescent dyes Lysosome-targetable Fluorescence probe PEROXYNITRITE Acute liver injury 

分 类 号：O657.3[理学—分析化学] R575[理学—化学]