Dissimilarity of different cephalosporins on volatile fatty acids production and antibiotic resistance genes fates during sludge fermentation and underlying mechanisms  

作　　者：Jingyang Luo Yuxiao Li Wenxuan Huang Feng Wang Shiyu Fang Xiaoshi Cheng Qian Feng Fang Fang Jiashun Cao Yang Wu 

机构地区：[1]Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes,Ministry of Education,Hohai University,Nanjing 210098,China [2]College of Environment,Hohai University,Nanjing 210098,China [3]State Key Laboratory of Pollution Control and Resource Reuse,School of Environmental Science and Engineering,Tongji University,Shanghai 200092,China

出　　处：《Chinese Chemical Letters》2023年第4期396-401,共6页中国化学快报（英文版）

基　　金：financially supported by the National Natural Science Foundation of China (No. 52070069);Jiangsu Province Natural Science Foundation (No. BK20211207);China Postdoctoral Science Foundation (No. 2021M692423);Shanghai Post-doctoral Excellence Program (No. 2020419);the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD),China。

摘　　要：The distinct influences of cephalosporins(CEPs, i.e., cefamandole nafate and cefpirome sulfate) affiliated to different generations on the volatile fatty acids(VFAs) production and antibiotic resistance genes(ARGs) fates during waste activated sludge(WAS) fermentation were unveiled. The presence of CEPs mainly exhibited negative effects on the total VFAs production(5%–15% reduction), especially the cefamandole nafate, which is quite different to previous understanding. Further investigation revealed that the CEPs contributed to the solubilization and hydrolysis but inhibited the acidification process by affecting the functional microbial populations(i.e., Tissierella) and general microbial metabolic activities(i.e., pyruvate metabolism and VFAs biosynthesis). In addition, CEPs(especially the cefpirome sulfate)caused the propagation of ARGs(i.e., bla TEM, tet X and mex F) during WAS fermentation. CEPs enhanced the cell membrane permeability to promote the antibiotics mechanism of efflux pump and the horizontal transfer of ARGs. Also, the CEPs altered the regulatory systems(i.e., two component system) and microbial populations associated with ARGs, resulting in the proliferation of specific ARGs. Overall, the dissimilarity of different CEPs impacts on the WAS fermentation for VFAs production and ARGs variations enlightened the diverse environmental behaviors of anthropogenic pollutants and evoked the caution of ecological risks.

关 键 词：Wasteactivated sludge(WAS) Cephalosporins(CEPs) Volatilefattyacids(VFAs) Antibiotic resistancegenes(ARGs) Geneticexpressions 

分 类 号：TQ920.6[轻工技术与工程—发酵工程] X703[环境科学与工程—环境工程]