绞股蓝中总皂苷含量测定方法  

Determination of Gypenosides in Gynostemma pentaphyllum

作　　者：许芳瑞 刘新艳 刘茜 杨宇 赵浩安 曹炜 XU Fangrui;LIU Xinyan;LIU Qian;YANG Yu;ZHAO Haoan;CAO Wei(College of Food Science and Technology,Northwest University,Xi’an 710069;Xi’an Xida Life Science and Health Research Institute Co.,Ltd.,Xi’an 710069)

机构地区：[1]西北大学食品科学与工程学院,西安710069 [2]西安西大生命科学与健康研究院有限公司,西安710069

出　　处：《食品工业》2023年第4期138-141,共4页The Food Industry

基　　金：大学生创新创业训练计划项目(项目编号:202210697040);陕西省重点研发计划-重点产业创新链(群)(项目编号:2023-ZDLNY-30)。

摘　　要：建立比色法测定绞股蓝中总皂苷含量的方法。采用75%乙醇热回流提取绞股蓝中总皂苷,通过D101大孔吸附树脂纯化提取液,采用香草醛-高氯酸法比色体系测定绞股蓝中的总皂苷含量。结果表明,人参皂苷Rb1在550 nm处有最大吸收,在0.04~0.25 mg有良好的线性范围,相关系数r^(2)=0.9992,平均加样回收率为102.65%,相对标准偏差(SRSD)为2.77%(n=6)。采用D-101大孔吸附树脂提取纯化绞股蓝皂苷的方法简单、准确、灵敏度高、重复性好,可用于绞股蓝中总皂苷含量的测定。Establish a colorimetric method for the determination of total saponins in Gynostemma pentaphyllum.The total saponins in Gynostemma pentaphyllum were extracted by 75%ethanol and purified by D101 macroporous adsorption resin.The content of total saponins in Gynostemma pentaphyllum was determined by vanillin perchloric acid colorimetry.The results showed that ginsenoside Rb1 had a maximum absorption at 550 nm,with a good linear range of 0.04-0.25 mg,the correlation coefficient R^(2)was 0.9992,and the average sample recovery rate was 102.65%as well as the relative standard deviation(SRSD)was 2.77%(n=6).Extracting and purifying Gynostemma saponins by D-101 macroporous adsorption resin was simple and accurate,and had high sensitivity and good repeatability,which could be used for the determination of total saponins in Gynostemma pentaphyllum.

关 键 词：绞股蓝总皂苷 比色法 含量测定 人参皂苷RB1 

分 类 号：R284.1[医药卫生—中药学]