Identification of key genes involved in flavonoid and terpenoid biosynthesis and the pathway of triterpenoid biosynthesis in Passiflora edulis  

作　　者：XU Yi HUANG Dong-mei MA Fu-ning YANG Liu WU Bin XING Wen-ting SUN Pei-guang CHEN Di XU Bing-qiang SONG Shun 

机构地区：[1]National Key Laboratory for Tropical Crop Breeding/Haikou Experimental Station,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Genetic Improvement of Bananas,Sanya Research Institute of Chinese Academy of Tropical Agricultural Sciences,Sanya 572000,P.R.China [2]Hainan Yazhou Bay Seed Laboratory,Sanya 572000,P.R.China [3]Guangxi Crop Genetic Improvement and Biotechnology Laboratory,Guangxi Academy of Agricultural Sciences,Nanning 530007,P.R.China

出　　处：《Journal of Integrative Agriculture》2023年第5期1412-1423,共12页农业科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (32260737);the Project of Sanya Yazhou Bay Science and Technology City, China (SCKJJYRC-2022-84 and SCKJ-JYRC-2022-93);the Hainan Provincial Natural Science Foundation of China (320QN305, 321MS091 and 320RC686)。

摘　　要：Passion fruit(Passiflora edulis Sims) is a vine of the Passiflora genus in the Passifloraceae family. The extracted components include flavonoids and terpenoids, which have good anti-anxiety and anti-inflammatory effects in humans.In this study, we analyzed the transcriptomes of four tissues of the ‘Zixiang’ cultivar using RNA-Seq, which provided a dataset for functional gene mining. The de novo assembly of these reads generated 96 883 unigenes, among which 61 022 unigenes were annotated(62.99% yield). In addition to its edible value, another important application of passion fruit is its medicinal value. The flavonoids and terpenoids are mainly derivatives of luteolin, apigenin, cycloartane triterpenoid saponins and other active substances in leaf extracts. A series of candidate unigenes in the transcriptome data that are potentially involved in the flavonoid and terpenoid synthesis pathways were screened using homologybased BLAST and phylogenetic analysis. The results showed that the biosynthesis of triterpenoids in passion fruit comes from the branches of the mevalonate(MVA) and 2-C-methyl-D-erythritol 4-phosphate/1-deoxy-D-xylulose 5-phosphate(MEP/DOXP) pathways, which is different from the MVA pathway that is used in other fruit trees. Most of the candidate genes were found to be highly expressed in the leaves and/or flowers. Quantitative real-time PCR(qRT-PCR) verification was carried out and confirmed the reliability of the RNA-Seq data. Further amplification and functional analysis of these putative unigenes will provide additional insight into the biosynthesis of flavonoids and terpenoids in passion fruit.

关 键 词：PASSION FRUIT RNA-Seq gene mining FLAVONOIDS TERPENOIDS 

分 类 号：S667.9[农业科学—果树学]