Herbicidal activity and biochemical characteristics of the botanical drupacine against Amaranthus retroflexus L.  

作　　者：YU Hua-long TIAN Ci SHEN Rong-yan Zhao Han YANG Juan DONG Jin-gao ZHANG Li-hui MA Shu-jie 

机构地区：[1]College of Plant Protection,Hebei Agricultural University,Baoding 071001,P.R.China [2]State Key Laboratory of North China Crop Improvement and Regulation,Baoding 071001,P.R.China [3]College of Agronomy and Biotechnology,Hebei Normal University of Science&Technology,Qinhuangdao 066004,P.R.China

出　　处：《Journal of Integrative Agriculture》2023年第5期1434-1444,共11页农业科学学报（英文版）

基　　金：funded by the Provincial Natural Science Foundation of Hebei for Excellent Young Scholar, China (C2021204071);the Science and Technology Project of Hebei Education Department (QN2021079);the Key Research and Development Project of Hebei Province (21326511D and 19226504D);the China Agriculture Research System of MOF and MARA (CARS-02)。

摘　　要：Botanical herbicide has been a hot topic in the research and development of novel pesticides. The herbicidal activity and biochemical characteristics of the botanical compound drupacine were studied by evaluating its effects on seed germination, seedling growth, morphological and physiological characteristics of Amaranthus retroflexus. Drupacine inhibited seed germination and seedling growth, and had a median inhibition concentration(IC50) value of 38.99 mg L-1against A. retroflexus root. The α-amylase activity and soluble sugar content in treated plants were significantly lower than that of the control. The expression of α-amylase gene was dosage-dependently inhibited compared to the untreated control. This suggested that inhibition of α-amylase activity was a mode of action on seed germination. The root hairs were significantly decreased and part of the root cap fell off after treatment with drupacine. The ultrastructure observation showed that cell damage of root tips increased with the treatment time. Drupacine also increased the relative conductivity and malondialdehyde(MDA) content. Peroxidase(POD), catalase(CAT), and superoxide dismutase(SOD) activities were significantly enhanced in the treatment compared to the control. These findings indicated that the physiological and biochemical reaction changes leading to morphological and membrane injuries were the main effects of drupacine on the inhibition of seedling growth. Drupacine can be developed as a botanical herbicide.

关 键 词：drupacine herbicidal ACTIVITY PHYSIOLOGICAL CHARACTERISTIC DEFENSE capacity AMARANTHUS retroflexus 

分 类 号：TQ457[化学工程—农药化工]