饲料中添加红景天对凡纳滨对虾抗氧化功能及盐度骤变适应性的影响  

Effects of Dietary Rhodiola rosea on Antioxidant System Function and Salinity Sudden Change Adaptation of Litopenaeus vannamei

作　　者：周文川 王芸[2] 段亚飞[2] 王珺[2] 周传朋[2] 黄忠[2] ZHOU Wenchuan;WANG Yun;DUAN Yafei;WANG Jun;ZHOU Chuanpeng;HUANG Zhong(Shenzhen Fisheries Development Center,Shenzhen,Guangdong 518067;South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences/Key Laboratory of Aquatic Product Processing,Ministry of Agriculture and Rural Affairs/Guangdong Provincial Key Laboratory of Fishery Ecology and Environment,Guangzhou 510300)

机构地区：[1]深圳市渔业发展研究中心,广东深圳518067 [2]中国水产科学研究院南海水产研究所/农业农村部水产品加工重点实验室/广东省渔业生态环境重点实验室,广州510300

出　　处：《中国农学通报》2023年第14期144-151,共8页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金项目“凡纳滨对虾Nrf2在绿原酸诱导GPx基因转录的功能研究”(31502183);中央级公益性科研院所基本科研业务费专项资金(中国水产科学研究院南海水产研究所)资助项目“MST调控凡纳滨对虾抗氧化功能机制的研究”(2020XK03);广东省科技计划项目“解淀粉芽孢杆菌发酵马尾藻的高值化综合利用”(2015A020209044)。

摘　　要：研究旨在分析饲料中添加红景天(Rhodiola rosea)对凡纳滨对虾(Litopenaeus vannamei)抗氧化功能及盐度骤变适应性的影响。将0、0.3、1、3 g/kg红景天分别添加到基础饲料,饲喂凡纳滨对虾28 d,然后进行低盐度(10)骤变实验,分析凡纳滨对虾血清T-AOC、GSH-Px、CAT活力及血细胞GSH-Px、CAT基因表达水平。双因素方法分析结果显示,红景天添加量和养殖与盐度骤变时间对血清GSH-Px、CAT活力和血细胞GSH-Px、CAT基因表达水平影响显著(P<0.001),且红景天添加量和养殖与盐度骤变时间的交互作用对凡纳滨对虾T-AOC、GSH-Px、CAT活力和GSH-Px、CAT基因表达水平的影响均有统计学意义。简单效应分析结果显示饲料中添加0.3 g/kg红景天时,养殖与盐度骤变时间对凡纳滨对虾血清TAOC活力影响最小(F=2.338,P<0.001);饲料中添加0.3 g/kg红景天时,养殖与盐度骤变时间对凡纳滨对虾血清GSH-Px(F=5696.236,P<0.001)、CAT(F=0.314,P<0.001)酶活力和血细胞GSH-Px (F=0.524,P<0.001)、CAT基因表达水平(F=0.336,P<0.001)相对影响较小。饲料中添加0.3 g/kg红景天在凡纳滨对虾养殖与盐度骤变适应性中维持抗氧化防御系统的稳定状态更为合适。To investigate the effect of Rhodiola rosea extract on antioxidant system function and salinity change adaptation of Litopenaeus vannamei,four diets were prepared with R.rosea inclusion at 0(control diet),0.3,1 and 3 g/kg and fed to shrimp for 28 days.Then low salinity(10)change adaptation test was conducted and antioxidant system function including total antioxidant status(T-AOC),glutathione peroxidase(GSH-Px),catalase(CAT)in serum as well as gene expression of GSH-Px and CAT in hemocyte of shrimp were analyzed.Two-way ANOVA analysis results showed that R.rosea extract levels and times(rearing and salinity change time)both played a significant role on the effect of GSH-Px and CAT activities in serum and gene expression levels of GSH-Px and CAT in hemocyte of shrimp(P<0.001).There was a significant interaction between the effects of two factors(R.rosea extract level&rearing and salinity sudden change times)on the activities of T-AOC,GSH-Px,CAT and gene expression levels of GSH-Px and CAT.Simple effect analysis indicated that the effect of rearing and salinity change times on T-AOC activity in serum of shrimp was the lowest when 0.3 g/kg R.rosea extract was added into feed(F=2.338,P<0.001).Moreover,the effects of rearing and salinity change times on the activities of GSH-Px(F=5696.236,P<0.001),CAT(F=0.314,P<0.001)in serum and gene expression levels of GSH-Px and CAT in hemocytes were relatively low.Diets containing 0.3 g/kg R.rosea extract are more suitable for maintaining the stability in the antioxidant defense system of L.vannamei and its adaptability to salinity sudden change.

关 键 词：红景天 凡纳滨对虾 抗氧化功能 盐度骤变 

分 类 号：S9[农业科学—水产科学]