再生丝素蛋白膜表面的纳米图案化修饰对细胞黏附和增殖的影响  

Effects of Surface Nano-Pattern Modification of Regenerated Silk Fibroin Film on Cell Adhesion and Proliferation

作　　者：欧阳秦君 刘晓娇 姚响 张耀鹏[1] Ouyang Qinjun;Liu Xiaojiao;Yao Xiang;Zhang Yaopeng(College of Materials Science and Engineering,State Key Laboratory for Modification of Chemical Fibers and Polymer Materials,Donghua University,Shanghai 201620,China)

机构地区：[1]纤维材料改性国家重点实验室,东华大学材料科学与工程学院,上海201620

出　　处：《中国生物医学工程学报》2023年第2期201-209,共9页Chinese Journal of Biomedical Engineering

基　　金：上海市自然科学基金(20ZR1402400);中央高校基本科研业务费专项基金(2232020D-04);国家自然科学基金(52273125)。

摘　　要：为了拓展再生丝素蛋白(RSF)生物医用材料在细胞培养和组织修复等生物医学领域中的应用,提高RSF材料的细胞相容性至关重要。本研究采用等离子体刻蚀技术制备了纳米“岛”图案化RSF膜,并进一步考察其对NIH3T3细胞黏附和增殖行为的影响。所有定量检测实验中至少设3个重复样(n≥3)。细胞培养1 d后的显微观察结果表明,与未修饰的RSF平整膜相比,纳米图案化修饰的RSF膜具有更好的黏附形态。CCK-8检测细胞培养1和4 d的吸光度(OD)结果表明,与未修饰的RSF平整膜相比,纳米图案化修饰RSF膜能够明显促进细胞的增殖,且其中刻蚀30 min膜组的吸光度(1.13±0.32)最高,显著高于未修饰的RSF平整膜(0.46±0.03,P<0.001)。等离子体刻蚀技术可作为一种简便、快捷、可大规模制备的纳米图案化修饰策略用于提高RSF材料的细胞相容性。Improving the cytocompatibility of regenerated silk fibroin(RSF)biomaterials is very important to expand its application in the biomedical fields of cell culture and tissue repair.This study prepared three kinds of special nano-island-patterned RSF films by plasma etching with different etching times,and further investigated their effects on the adhesion and proliferation of NIH3T3 cells,and all quantification experiments were set at least three replicates(n≥3).The micrograph observation results after 1 d of cell culture showed that cells on the nano-pattern modified RSF films had better adhesion morphology when compared to those on the non-modified flat RSF film.The results of OD value detected by CCK-8 after 1 and 4 d of cell culture showed that nano-pattern modified RSF films significantly promoted the cell proliferation compared to the nonmodified flat RSF film.Moreover,cells on the 30 min-etched film presented the highest OD value(OD=1.13±0.32),which was much higher than that of on the non-modified flat RSF film(OD=0.46±0.03,P<0.001).In summary,plasma etching provided a simple,rapid,and large-scale nano-pattern modification strategy to improve the cytocompatibility of RSF materials,which is expected to provide important references and guidance for the surface modification of RSF and other biomaterials.

关 键 词：再生丝素蛋白 等离子体刻蚀 纳米图案化修饰 细胞黏附 细胞增殖 

分 类 号：R318[医药卫生—生物医学工程]