长链非编码RNA小核仁RNA宿主基因22靶向作用于微小RNA-27a-3p/胰岛素样生长因子-1信号轴对三阴性乳腺癌细胞侵袭能力的影响  

Effect of long non coding RNA small nucleolar RNA host gene 22 targeting microRNA-27a-3p/insulin-like growth factor-1 axis on the invasion of triple negative breast cancer cells

作　　者：李筱雨[1] 申伟[1] 赵苏远[1] 张晓旭 闫贺一 冀宏[1] Li Xiaoyu;Shen Wei;Zhao Suyuan;Zhang Xiaoxu;Yan Heyi;Ji Hong(Department of Thyroid and Breast Surgery,the Second Hospital of Hebei Medical University,Shijiazhuang 050000,China;Graduate School of Hebei Medical University,Shijiazhuang 050000,China)

机构地区：[1]河北医科大学第二医院甲状腺乳腺外科,石家庄050000 [2]河北医科大学研究生学院,石家庄050000

出　　处：《中华实验外科杂志》2023年第4期615-618,共4页Chinese Journal of Experimental Surgery

摘　　要：目的观察三阴性乳腺癌细胞中长链非编码RNA小核仁RNA宿主基因22(SNHG22)靶向作用于微小RNA(miR)-27a-3p/胰岛素样生长因子-1对细胞侵袭能力的影响。方法选取2019年6月至2022年3月河北医科大学第二医院甲状腺乳腺外科收治30例三阴性乳腺癌及其癌旁组织标本作为研究对象,采用荧光定量聚合酶链反应(PCR)分析癌及癌旁组织中SNHG22和miR-27a-3p的表达变化;将SNHG22过表达质粒和miR-27a-3p模拟物(mimics)分别转染至三阴性乳腺癌MDA-MB-231细胞中,利用细胞侵袭实验观察两者对细胞侵袭能力的影响;利用生物信息学软件及双荧光素酶报告基因分析SNHG22和miR-27a-3p的关系及miR-27a-3p的下游靶基因。将miR-27a-3p mimics、miRNA阴性对照(miR-NC)分别与SNHG22共转染后,观察上调miR-27a-3p表达对过表达SNHG22的MDA-MB-231细胞侵袭能力的影响。两组间比较采用t检验。结果荧光定量PCR实验结果显示,SNHG22在三阴性乳腺癌组织中的表达水平(3.184±1.800)明显高于癌旁组织(1.295±0.659,t=5.395,P<0.05);miR-27a-3p的表达水平(0.547±0.414)明显低于癌旁组织(1.521±0.845,t=5.623,P<0.05);三阴性乳腺癌细胞中过表达SNHG22组侵袭细胞数(406.500±9.492)明显高于对照组(172.000±14.142,t=67.000,P<0.05),而转染miR-27a-3p mimics转染组侵袭细胞数(139.000±19.789)明显低于对照组(391.000±21.213,t=252.000,P<0.05);生物信息学预测及荧光素酶报告基因分析结果表明,miR-27a-3p可与SNHG22结合降低细胞的荧光素酶活性(0.415±0.054比1.014±0.106,t=16.360,P<0.05),而miR-27a-3p可与IGF-1的3’端非编码区(3’UTR)结合降低细胞的荧光素酶活性(0.367±0.049比1.015±0.021,t=12.860,P<0.05);挽救实验结果表明SNHG22+miR-27a-3p mimics组侵袭细胞数(186.500±14.849)明显低于SNHG22+miR-NC组细胞(345.000±24.061),差异有统计学意义(t=24.380,P<0.05)。结论长链非编码RNA SNHG22可通过靶向作用于miR-27a-3p/IGF-1调控三阴性乳腺癌细胞的Objective To observe the effect of long non-coding RNA small nucleolar RNA host gene 22(SNHG22)targeting microRNA(miR)-27a-3p/insulin-like growth factor-1(IGF-1)axis on cell invasion in triple negative breast cancer cells.Methods A total of 30 cases of triple negative breast cancer and adjacent tissues collected in the Second Hospital of Hebei Medical University from June 2019 to March 2022 were selected as the research objects.Real-time polymerase chain reaction(PCR)method was used to observe the expression level of SNHG22 and miR-27a-3p in triple negative breast cancer and adjacent tissues.The SNHG22 overexpression plasmid and miR-27a-3p mimics were transfected into triple negative breast cancer MDA-MB-231 cells,respectively,and the effect of SNHG22 and miR-27a-3p on the invasion ability of triple negative breast cancer cells was observed by the cell invasion experiment.The relationship between SNHG22 and miR-27a-3p and the downstream target gene of miR-27a-3p was analyzed using bioinformatics software and double luciferase reporter gene.After co-transfection of miR-27a-3p mimics and miRNA negative control(miR-NC)with SNHG22,the effect of up-regulation of miR-27a-3p expression on the invasion ability of MDA-MB-231 cells overexpressing SNHG22 was observed.T-test was used to compare the two groups.Results The results of real-time PCR experiment indicated that the expression level of SNHG22 in triple negative breast cancer tissues(3.184±1.800)was significantly higher than that in adjacent tissues(1.295±0.659,t=5.395,P<0.05),and the expression level of miR-27a-3p(0.547±0.414)was obviously lower than that of adjacent tissues(1.521±0.845,t=5.623,P<0.05).The number of invasive cells was significantly increased after transfection with SNHG22 overexpression plasmid(406.500±9.492)as compared with that in the control group(172.000±14.142,t=67.000,P<0.05),while the number of invasive cells in miR-27a-3p mimics transfection group(139.000±19.789)was significantly reduced as compared with that in the control group(391.

关 键 词：乳腺癌 微小RNA 胰岛素样生长因子-1 侵袭 

分 类 号：R737.9[医药卫生—肿瘤]