罗哌卡因对骨肉瘤细胞增殖、侵袭及雷帕霉素靶蛋白/缺氧诱导因子1α信号通路的影响  

Effects of ropivacaine on proliferation,invasion and mTOR/HIF-1αsignaling pathway of osteosarcoma cells

作　　者：张素冰[1] 高辉[2] 赵滨滨[1] 张丹琦[1] ZHANG Subing;GAO Hui;ZHAO Binbin;ZHANG Danqi(Department of Anesthesiology and Surgery,First Affiliated Hospital,Heilongjiang University of Chinese Medicine,Harbin 150040,China)

机构地区：[1]黑龙江中医药大学附属第一医院麻醉手术科,黑龙江哈尔滨150040 [2]黑龙江中医药大学附属第三医院麻醉手术科,黑龙江哈尔滨150040

出　　处：《陕西医学杂志》2023年第6期666-670,共5页Shaanxi Medical Journal

基　　金：黑龙江省中医药科研项目(ZHY2020-129)。

摘　　要：目的:探究罗哌卡因对骨肉瘤细胞增殖、侵袭及雷帕霉素靶蛋白(mTOR)/缺氧诱导因子1α(HIF-1α)信号通路的影响。方法:将MG63细胞分为对照组、罗哌卡因低浓度组、罗哌卡因中浓度组、罗哌卡因高浓度组。采用平板克隆法测定MG63细胞平板克隆率,采用免疫组化法测定MG63细胞Ki-67表达,采用Transwell法测定MG63细胞侵袭、迁移能力,采用Western blot法测定MG63细胞基质金属蛋白酶2(MMP-2)、MMP-9、mTOR、HIF-1α蛋白表达。结果:与对照组比较,罗哌卡因低、中、高浓度组MG63细胞克隆形成率、Ki-67阳性率、侵袭细胞数、迁移细胞数以及MMP-2、MMP-9、mTOR、HIF-1α蛋白表达降低(均P<0.05)。与罗哌卡因低浓度组比较,罗哌卡因中、高浓度组MG63细胞克隆形成率、Ki-67阳性率、侵袭细胞数、迁移细胞数以及MMP-2、MMP-9、mTOR、HIF-1α蛋白表达降低(均P<0.05)。与罗哌卡因中浓度组比较,罗哌卡因高浓度组MG63细胞克隆形成率、Ki-67阳性率、侵袭细胞数、迁移细胞数以及MMP-2、MMP-9、mTOR、HIF-1α蛋白表达降低(均P<0.05)。结论:罗哌卡因可抑制MG63细胞增殖、侵袭、迁移,并可抑制mTOR/HIF-1α通路的激活。Objective:To investigate the effects of ropivacaine on the proliferation,invasion and mammalian target of rapamycin(mTOR)/hypoxia-inducible factor 1α(HIF-1α)signaling pathway of osteosarcoma cells.Methods:MG63 cells were grouped into control group,low-concentration ropivacaine group,medium-concentration ropivacaine group,and high-concentration ropivacaine group.Plate cloning method was applied to determine the plate cloning rate of MG63 cells.Immunohistochemistry was used to determine the expression of Ki-67 in MG63 cells.Transwell method was applied to determine the invasion and migration abilities of MG63 cells.Western blot was applied to determine the protein expressions of Matrix metalloproteinase 2(MMP-2),MMP-9,mTOR and HIF-1αin MG63 cells.Results:Compared with the control group,the colony formation rate,the positive rate of Ki-67,the number of invasive cells,the number of migrating cells,the protein expressions of MMP-2,MMP-9,mTOR and HIF-1αdecreased in the low-,medium-and high-concentration ropivacaine group(all P<0.05).Compared with the low-concentration ropivacaine group,the colony formation rate,the positive rate of Ki-67,the number of invasive cells,the number of migrating cells,the protein expressions of MMP-2,MMP-9,mTOR and HIF-1αdecreased in the medium-and high-concentration ropivacaine group(all P<0.05).Compared with the medium-concentration ropivacaine group,the colony formation rate,the positive rate of Ki-67,the number of invasive cells,the number of migrating cells,the protein expressions of MMP-2,MMP-9,mTOR and HIF-1αdecreased in the high-concentration ropivacaine group(all P<0.05).Conclusion:Ropivacaine can inhibit the proliferation,invasion and migration of MG63 cells,and can inhibit the activation of mTOR/HIF-1αpathway.

关 键 词：骨肉瘤 罗哌卡因 增殖 侵袭 雷帕霉素靶蛋白 缺氧诱导因子1Α 

分 类 号：R738.1[医药卫生—肿瘤]