黑玉米芯花色苷提取工艺优化及其抗氧化特性研究  

Optimization of extraction process and antioxidant function of anthocyanins from black corncob

作　　者：陈浩 韩天祥 徐佳铭 刘文卓 李俊杰 赵哲 陆荣华 李先保 陈春旭 CHEN Hao;HAN Tianxiang;XU Jiaming;LIU Wenzhuo;LI Junjie;ZHAO Zhe;LU Ronghua;LI Xianbao;CHEN Chunxu(College of Food Engineering,Anhui Science and Technology University,Fengyang 233100,China;Anhui Weihong Foods Co.,Ltd.,Bengbu 233000,China)

机构地区：[1]安徽科技学院食品工程学院,安徽凤阳233100 [2]安徽伟宏食品有限公司,安徽蚌埠233000

出　　处：《安徽科技学院学报》2023年第3期69-75,共7页Journal of Anhui Science and Technology University

基　　金：国家自然科学基金(31901617);国家级大学生创新创业训练计划项目(202210879093,202110879047);安徽省高校优秀青年人才支持计划重点项目(gxyqZD2021125);安徽科技学院优秀人才项目(XJYXRC202101);安徽科技学院稳定人才项目(SPWD202101)。

摘　　要：目的:研究黑玉米芯花色苷的最优提取工艺及其抗氧化活性。方法:以提取时间、提取温度、乙醇添加量、黑玉米芯粉末与提取液的料液比为变量,花色苷含量为评价指标,利用响应面试验确定相关因素的最佳数值,并结合单因素试验进行验证,最终确定最佳提取工艺。此外,通过对自由基清除率的研究,评价黑玉米芯花色苷的抗氧化能力。结果:在最优提取工艺为料液比1∶13(m/V)、提取温度48.9℃、提取时间2.9 h、82%乙醇(含1%甲酸)添加量的条件下,黑玉米芯花色苷的含量约为(363.15±13.35)mg/L,且黑玉米芯花色苷具有显著的DPPH、ABTS自由基清除能力(P<0.05)。结论:在最佳提取条件下,提取液中黑玉米芯花色苷的含量较高,并且有较强的抗氧化能力,该研究对黑玉米芯花色苷的生物活性研究及应用具有一定的理论指导意义。Objective:To investigate the optimal extraction process of anthocyanin from black corncob and its antioxidant properties.Methods:The extraction time,extraction temperature,the content of ethanol,the material-to-liquid ratio were used as variables.In the meantime,the anthocyanin content of extraction solution was used to determine the optimal values of the relevant factors by response surface testing.In addition,the antioxidant capacity of black corncob anthocyanin was evaluated by the study of the free radical scavenging rate.Results:The optimal extraction process was listed as follows:black corncob powder∶extraction solution=1∶13(m/V),extraction temperature of 48.9℃,e xtraction time of 2.9 h,extraction solution of 82%ethanol solution containing 1%formic acid.The content of black corncob anthocyanin was about(363.15±13.35)mg/L.Meanwhile,black corncob anthocyanin had significant DPPH and ABTS free radical scavenging ability(P<0.05).Conclusion:Under the optimal extraction conditions,the high content of black corncob anthocyanin has a strong antioxidant ability.This study may provide certain theoretical guidance for the study of the biological activity of black corncob anthocyanin.

关 键 词：黑玉米芯 花色苷 提取工艺 抗氧化 

分 类 号：TS201.1[轻工技术与工程—食品科学]