华蟾素注射液对胃癌细胞MGC-803增殖、迁移和侵袭能力及上皮-间质转化的影响  

Effect of Cinobufagin Injection on the Proliferation,Migration,and Invasion Abilities and Epithelial-Mesenchymal Transition of Gastric Cancer MGC803 Cells

作　　者：涂雅婷 王萌 程卉[1,2,3] 李庆林 TU Yating;WANG Meng;CHENG Hui;LI Qinglin(School of Pharmacy,Anhui University of Chinese Medicine,Anhui Hefei 230012,China;Key Laboratory of Xin an Medicine,Ministry of Education,Research and Technology Center,Anhui University of Chinese Medicine,Anhui Hefei 230038,China)

机构地区：[1]安徽中医药大学药学院,安徽合肥230012 [2]安徽中医药大学科研技术中心,安徽合肥230038 [3]新安医学教育部重点实验室,安徽合肥230038

出　　处：《安徽中医药大学学报》2023年第3期67-71,共5页Journal of Anhui University of Chinese Medicine

基　　金：国家自然科学基金项目(81673650,81903859)。

摘　　要：目的 探究华蟾素(cinobufagin,CBG)注射液对人胃癌细胞MGC803增殖、迁移和侵袭能力以及上皮-间质转化(epithelial-mesenchymal transition,EMT)的影响。方法 CCK-8检测CBG注射液对MGC-803存活率的影响;倒置显微镜下观察CBG注射液对MGC-803形态的影响;采用细胞划痕实验和Transwell小室检测CBG注射液对胃癌细胞迁移和侵袭能力的影响;Western blot检测人胃癌细胞MGC-803中E-cadherin、N-cadherin、Vimentin和Snail蛋白表达水平。结果 华蟾素注射液能显著抑制MGC-803细胞的增殖、迁移与侵袭(P<0.05),且呈剂量依赖性。Western blot检测结果显示,CBG注射液能显著上调E-cadherin蛋白表达水平(P<0.05),显著下调N-cadherin、Vimentin、Snail蛋白表达水平(P<0.05)。结论 华蟾素注射液可以抑制胃癌细胞MGC-803的增殖、迁移侵袭及EMT。Objective To investigate the effect of cinobufagin(CBG)injection on the proliferation,migration,and invasion abilities and epithelial-mesenchymal transition(EMT)of human gastric cancer MGC803 cells.Methods CCK-8 assay was used to observe the effect of CBG injection on the survival rate of MGC803 cells;the effect of CBG injection on the morphology of MGC803 cells was observed under an inverted microscope;wound healing assay and Transwell assay were used to investigate the effect of CBG injection on the migration and invasion abilities of gastric cancer cells;Western blot was used to measure the protein expression levels of E-cadherin,N-cadherin,Vimentin,and Snail in human gastric cancer MGC803 cells.Results CBG injection significantly inhibited the proliferation,migration,and invasion of MGC803 cells in a dose-dependent manner.Western blot showed that CBG significantly upregulated the protein expression level of E-cadherin(P<0.05)and downregulated the protein expression levels of N-cadherin,Vimentin,and Snail(P<0.05).Conclusion CBG injection can inhibit the proliferation,migration,invasion,and EMT of gastric cancer MGC803 cells.

关 键 词：华蟾素注射液 胃癌细胞 侵袭 转移 上皮-间质转化 

分 类 号：R735.2[医药卫生—肿瘤]