油莎豆生长素受体TIR1基因家族鉴定及响应盐胁迫和外源IBA的表达分析  

Identification of Auxin Receptor Gene TIR1 Family in Cyperus esculentus and the Expression Analysis in Response to Salt Stress and Exogenous IBA

作　　者：李昕儒 高宇 苗淑楠 李腾 董书言 史先飞 薛金爱[1] 季春丽 李润植[1] LI Xinru;GAO Yu;MIAO Shunan;LI Teng;DONG Shuyan;SHI Xianfei;XUE Jin’ai;JI Chunli;LI Runzhi(College of Agriculture,Shanxi Agricultural University,Taigu,Shanxi 030801,China)

机构地区：[1]山西农业大学农学院,山西太谷030801

出　　处：《热带作物学报》2023年第5期894-904,共11页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金项目(No.31902344);山西省高等学校科技创新项目(No.2019L0376);山西省基础研究项目(No.20210302124170)。

摘　　要：特色油料作物油莎豆(Cyperus esculentus L.)地下块茎可积累大量油脂,且具有适应性广和抗逆性强等特点,是研究植物抗逆性和地下营养器官富集油脂的优异材料。运输抑制剂响应蛋白1(transport inhibitor response protein 1,TIR1)作为生长素受体可调控植物生长发育和响应非生物胁迫。然而,油莎豆CeTIR1的相关研究鲜有报道。本研究基于油莎豆转录组数据筛选鉴定出4个油莎豆TIR1基因(CeTIR1-1、CeTIR1-2、CeTIR1-3和CeTIR1-4),并采用RT-PCR技术克隆到4个CeTIR1成员的ORFs。生物信息学分析表明,4个CeTIR1蛋白含有典型的F-box蛋白结构域、AMN1超结构域和Transp_inhibit结构域。CeTIR1s序列长度、编码蛋白相对分子量和等电点等理化性质差异较小。4个CeTIR1分别与禾本目莎草科、木犀科及十字花科的植物来源的TIR1s在进化上亲缘关系较近。基因表达分析显示,油莎豆CeTIR1基因在不同组织表达水平差异显著,均在块茎组织中高表达,在根和叶组织中表达量较低。盐胁迫处理下油莎豆幼苗抗氧化酶活性和MDA含量升高,添加外源IBA处理后,盐胁迫油莎豆幼苗的抗氧化酶活性进一步升高且MDA含量降低。这预示着IBA可以缓解盐胁迫对油莎豆幼苗生长的不利影响。qRT-PCR结果显示,4个CeTIR1基因在盐胁迫处理幼苗中表达量增加,外源添加IBA亦可显著上调CeTIR1-2基因的表达。综合分析显示CeTIR1-2可能是参与调控油莎豆幼苗响应盐胁迫的一个重要基因。研究结果可为挖掘生长素调控油莎豆生长发育和抗逆性相关功能基因以及油莎豆逆境栽培研究提供科学依据和重要参考。Cyperus esculentus is a special oil crop,with its underground tuber accumulating a large amount of oil,and also having the advantages of wide adaptability and strong stress resistance.This crop is a superior germplasm material for studying plant stress resistance and oil enrichment in the underground vegetative organs.Transport inhibitor response protein 1(TIR1)can regulate plant growth and development and abiotic stress response as an auxin receptor.However,few reports on CeTIR1 have been reported.In this study,four TIR1 genes,namely CeTIR1-1,CeTIR1-2,CeTIR1-3 and CeTIR1-4,were identified from C.esculentus transcriptome data and the ORFs were further cloned by RT-PCR.All the four CeTIR1 proteins contained a typical F-box protein domain,AMN1 superdomain and Transp_inhibit domain,revealed by bioinformatics analysis.No significant difference was detected among the four CeTIR1 proteins in regarding sequence length,relative molecular weight,isoelectric point and other physicochemical properties.The four CeTIR1s were closely related to TIR1s derived from plants of Cyperaceae,Oleaceae and Cruciferae in the evolution.Gene expression profiling showed that the expression levels of CeTIR1 family genes were significantly different in different tissues of C.esculentus,with the highest expression in tuber tissues and lower expression in root and leaf tissues.The antioxidant enzyme activity and MDA content of C.esculentus seedlings increased under salt stress.After adding exogenous IBA treatment,the antioxidant enzyme activity of C.esculentus seedlings under salt stress further increased whereas the MDA content decreased.This indicated that IBA could alleviate the adverse effects of salt stress on the growth of C.esculentus seedlings.qRT-PCR results showed that expression levels of the four CeTIR1 genes increased in C.esculentus seedlings treated with salt stress,and exogenous addition of IBA could significantly up-regulate the expression of CeTIR1-2 gene.Comprehensive analysis demonstrates that CeTIR1-2 may be an important ge

关 键 词：油莎豆 运输抑制剂响应蛋白1(TIR1) 吲哚丁酸(IBA) 盐胁迫 基因表达 

分 类 号：S565.9[农业科学—作物学]