不同产地龙脑樟枝叶化学成分分析及体外抑菌研究  

Chemical Composition Analysis and in vitro Bacteriostasis Research of Cinnamomum camphora(L.)Presl Branches and Leaves From Difer­ent Habitats

作　　者：李思琪 江芊[2] 黄英 胡璇[2] 王凯[2] 官玲亮[2] 于福来[2] 庞玉新 LI Siqi;JIANG Qian;HUANG Ying;HU Xuan;WANG Kai;GUAN Lingliang;YU Fulai;PANG Yuxin(School of Chinese Materia Medica Resources,Guangdong Pharmaceutical University,Yunfu,Guangdong 527300,China;Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Biology and Cultivation of Herb Medicine(Haikou),Ministry of Agriculture and Rural Affairs/Hainan Provincial Engineering Research Center for Tropical Medicinal Plants,Haikou,Hainan 571101,China;School of Pharmacy,Guizhou University of Chinese Medicine,Guiyang,Guizhou 550025,China)

机构地区：[1]广东药科大学中药资源学院,广东云浮527300 [2]中国热带农业科学院热带作物品种资源研究所/农业农村部中药材生物学与栽培重点实验室/海南省热带药用植物工程研究中心,海南海口571101 [3]贵州中医药大学药学院,贵州贵阳550025

出　　处：《热带作物学报》2023年第5期1011-1022,共12页Chinese Journal of Tropical Crops

基　　金：广东省科研事业单位(省实验室分中心)重点领域研发计划项目(No.2021B0707010008);广东省重点领域研发计划项目(No.2020B020221002)。

摘　　要：龙脑樟为目前国内提取天然冰片效益最高的植物,为助力其道地产区的确定,本研究根据龙脑樟的习性,拟选取国内8个产地的龙脑樟枝叶为材料,采用水蒸汽蒸馏法分别提取天然冰片;通过气质联用法(GC-MS)测定其中化学成分的相对含量;通过纸片法测定其对大肠杆菌、伤寒沙门氏菌以及肠炎沙门氏菌的抑菌圈直径;运用96微量孔板测定最小抑菌浓度(MIC)和最小杀菌浓度(MBC)。结果表明:各个产地龙脑樟枝叶提取的天然冰片中化合物含量具有差异性,其中以右旋龙脑、樟脑、d-柠檬烯、α-葎草烯、4-萜烯醇、桉叶油醇、莰烯以及d-α-蒎烯等为共有成分,且以右旋龙脑含量最高,樟脑次之,各产地中以湖北远安的右旋龙脑含量最高(82.47%),以广东乳源的右旋龙脑含量最低(41.63%);各个产地提取的天然冰片对3个供试菌种均有一定抑菌作用,且抑菌圈直径范围为14.60~27.95 mm;最低抑菌浓度(MIC)范围为250~1250μg/mL;最低杀菌浓度(MBC)范围为0~1000μg/mL,总体以湖北远安和江西吉水的综合抑菌效果最佳,广东乳源最差。由此可见,右旋龙脑含量最高的产地抑菌效果最佳,反之抑菌效果欠佳,因此,龙脑樟枝叶提取物的抑菌活性与其主要成分含量有一定相关性,本研究为龙脑樟枝叶提取物的品质评价及天然冰片的资源开发提供依据。C.camphora(L.)Presl is the most effective plant for extracting natural borneol in China.In order to help determine its proper habitat,this research plans to select branches and leaves of C.camphora(L.)Presl from eight ha-bitats in China according to its habits,natural borneol of the leaves and branches of C.camphora(L.)Presl were ex-tracted by steam distillation.The relative content of the chemical components in the extract was determined by GC-MS.The bacteriostatic circle diameter of Escherichia coli,Salmonella typhi and Salmonella enteritidis subsp was measured by the paper method,the minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)were determined by 96 micro well plates.Chemical composition determination showed that the content of natural borneol extracted from the branches and leaves from different habitats was different,among them,d-borneol,camphor,d-limonene,eucalyptol,d-α-pinene,camphene,α-humulene and terpinen-4-ol were the common components,and the content of d-borneol was the highest,then camphor.The highest content of d-borneol was found from Yuan’an,Hubei,China(82.47%),and the lowest content was found from Ruyuan,Guangdong,China(41.63%).The natural borneol ex­tracted from various habitats showed bacteriostatic effect on the three tested strains,and the diameter range of bacterio­static ring was 14.60 to 27.95 mm,the minimum inhibitory concentration(MIC)ranged from 250 to 1250μg/mL,the minimum bactericidal concentration(MBC)ranged from 0 to 1000μg/mL.The natural borneol from Yuan’an and Jishui,Jiangxi had the best bacteriostatic effect,while that from Guangdong had the worst on the whole.In conclusion,the bacteriostatic activity of the extracts from the branches and leaves of C.camphora(L.)Presl is correlated with the con­tent of its main components,and the habitat with the highest content of d-bornol has the best bacteriostatic effect,while the other has poor bacteriostatic effect.The data obtained in this research are accurate and reliable,and would provide

关 键 词：龙脑樟 右旋龙脑 相对含量 抑菌活性 天然冰片 

分 类 号：R284.1[医药卫生—中药学]