Multiplex genome editing targeting soybean with ultra-low anti-nutritive oligosaccharides  

作　　者：Wenxin Lin Huaqin Kuang Mengyan Bai Xiaomeng Jiang Pengfei Zhou Yinghua Li Bo Chen Huarong Li Yuefeng Guan 

机构地区：[1]College of Resources and Environment,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China [2]Guangdong Provincial Key Laboratory of Plant Adaptation and Molecular Design,Guangzhou Key Laboratory of Crop Gene Editing,Innovative Center of Molecular Genetics and Evolution,School of Life Sciences,Guangzhou University,Guangzhou 510006,Guangdong,China [3]FAFU-UCR Joint Center for Horticultural Biology and Metabolomics,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China [4]College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China [5]Bioinformatics Department,School of Life Sciences and Technology,Tongji University,Shanghai 200092,China [6]KingAgroot CropScience Co.,Ltd.,Qingdao 266500,Shandong,China

出　　处：《The Crop Journal》2023年第3期825-831,共7页作物学报（英文版）

基　　金：supported by the Chuying Scholar Project in Fujian Province and the Innovative Research Project at Fujian Agriculture and Forestry University。

摘　　要：Soybean is the primary source of plant protein for humans.Owing to the indigestibility of the raffinose family of oligosaccharides(RFO),raffinose and stachyose are considered anti-nutritive factors in soybean seeds.Low-RFO soybean cultivars are generated by mutagenesis of RFO biosynthesis genes,but the carbohydrate profiles invite further modification to lower RFOs.This study employed a pooled multiplex genome editing approach to target four seed-specifically expressed genes mediating RFO biosynthesis,encoding three raffinose synthases(RS2,RS3,and RS4)and one stachyose synthase.In T1progeny,rs2/rs3 and rs4/sts homozygous double mutants and a rs2/rs3/rs4/sts quadruple mutant(rfo-4m)were characterized.The rs2/rs3 mutant showed reduced raffinose and stachyose contents,but the rs4/sts mutant showed only reduced stachyose in seeds.The RFO contents in the rfo-4m mutant were almost eliminated.Metabolomic analysis showed that the mutation of four RFO biosynthesis genes led to a shift of metabolic profile in the seeds,including the accumulation of several oligosaccharides-related metabolites.These mutants could contribute to precision breeding of soybean cultivars for soy food production.

关 键 词：SOYBEAN Genome editing Raffinose family of oligosaccharides RAFFINOSE STACHYOSE Precision breeding 

分 类 号：S565.1[农业科学—作物学]