茄子叶际固氮菌的分离鉴定·培养特性及抑菌活性  

Isolation,Identification,Cultural Characteristics and Antibacterial Activity of Nitrogen-fixing Bacteria from Phyllosphere of Solanum melongena L.

作　　者：幸翀[1] 郭青云[2] XING Chong;GUO Qing-yun(School of Medical and Information Engineering,Gannan Medical University,Ganzhou,Jiangxi 341000;College of Life Sciences,Gannan Normal University,Ganzhou,Jiangxi 341000)

机构地区：[1]赣南医学院医学信息工程学院,江西赣州341000 [2]赣南师范大学生命科学学院,江西赣州341000

出　　处：《安徽农业科学》2023年第10期1-4,共4页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(32160314)。

摘　　要：采用Ashby无氮培养基从茄子叶际分离得到1株具有高效固氮性能的自生固氮菌QZ-1,经16S rDNA序列鉴定,该菌株与克雷伯氏菌属各菌株同源性高达99%以上,初步鉴定为克雷伯氏菌(Klebsiella sp.),其在酵母粉为碳源、pH为5.0时生长最好。菌株QZ-1具有广谱抗真菌活性,对油菜菌核病、葡萄炭疽病、番茄灰霉病抑制效果可达40%以上。自生固氮菌QZ-1具有固氮和生防作用,可为进一步研发优良的叶际固氮菌肥提供微生物资源。A nitrogen-fixing bacteria strain QZ-1 in the phyllosphere of Solanum melongena L.was screened using Ashby nitrogen-free medium.By 16S rDNA sequence analysis,the homology between strain QZ-1 and Klebsiella isolated was up to 99%,thus,the isolated strain was identified as Klebsiella sp..The growth of strain QZ-1 was better with yeast powder as carbon source at pH 5.0.Strain QZ-1 had broad-spectrum antifungal activity,with an inhibitory effect of over 40%on Sclerotinia sclerotiorum,grape anthracnose and Botrytis cinerea.The autotrophic nitrogen fixing bacterium QZ-1 had nitrogen fixation and biocontrol effects,which could provide microbial resources for the further development of nitrogen-fixing bacteria fertilizer.

关 键 词：茄子 叶际固氮菌 克雷伯氏菌 分离鉴定 培养特性 抑菌活性 

分 类 号：S182[农业科学—农业基础科学]