基于JAK2/STAT3通路分析姜黄素对视网膜缺血-再灌注损伤大鼠视网膜小胶质细胞极化的影响  

Analysis of the effect of Curcumin on retinal microglia polarization of rats with retinal ischemia reperfusion injury via janus kinase 2/signal transducer and activator of transcription 3 pathway

作　　者：尹伊 徐莹 赵玉泽 王晨旭 肖培伦 李晓双[2] 王晓莉[2] 赵岩松[1] YIN Yi;XU Ying;ZHAO Yuze;WANG Chenxu;XIAO Peilun;LI Xiaoshuang;WANG Xiaoli;ZHAO Yansong(Department of Ophthalmology,the Affiliated Hospital of Weifang Medical University,Weifang 261041,Shandong Province,China;Weifang Medical University,Weifang 261053,Shandong Province,China)

机构地区：[1]潍坊医学院附属医院眼科,山东省潍坊市261041 [2]潍坊医学院,山东省潍坊市261053

出　　处：《眼科新进展》2023年第6期439-443,共5页Recent Advances in Ophthalmology

基　　金：山东省自然科学基金(编号:ZR2021MH351,ZR2020MH074);国家自然科学基金(编号:82071888);山东省中医药科技发展计划项目(编号:2019-0421);潍坊市科学技术发展计划项目(编号:2021GX057,2021YX039)。

摘　　要：目的探讨姜黄素(CUR)对视网膜缺血-再灌注损伤(RIRI)大鼠视网膜小胶质细胞极化的影响,并通过JAK2/STAT3通路探讨其机制,以期为CUR的临床应用提供理论依据。方法健康雄性成年Sprague Dawley大鼠36只,随机分为假手术(Sham)组、RIRI组与CUR组,每组各12只。RIRI组和CUR组大鼠右眼采用高眼压法建立RIRI模型,Sham组仅将针头刺入大鼠右眼前房,不升高眼压。CUR组大鼠于建模前30 min腹腔注射CUR(100 mg·kg^(-1)),RIRI组和Sham组大鼠腹腔注射等剂量生理盐水。造模后24 h,采用HE染色及免疫组织化学染色法观察各组大鼠视网膜形态及视网膜神经节细胞(RGC)数变化,Iba-1/CD16、Iba-1/Arg1免疫荧光双标法检测CUR对RIRI大鼠视网膜小胶质细胞极化的影响,免疫组织化学结合Western blot检测观察CUR对RIRI大鼠RGC及JAK2/STAT3通路信号分子表达的影响。结果HE染色及免疫组织化学染色结果显示,Sham组大鼠视网膜细胞排列整齐,神经节细胞层见大量Brn-3a^(+)细胞(即RGC);与Sham组相比,造模后24 h,RIRI组大鼠内层视网膜厚度显著增加、RGC数显著减少,而CUR组大鼠内层视网膜厚度较RIRI组显著变薄,RGC数显著多于RIRI组(均为P<0.05)。免疫荧光双标染色结果显示,RIRI组大鼠视网膜Iba-1^(+)CD16^(+)细胞(M1型小胶质细胞)数较Sham组显著增加,而CUR组大鼠视网膜Iba-1^(+)CD16^(+)细胞数显著少于RIRI组,但仍多于Sham组(均为P<0.05);RIRI组大鼠视网膜Iba-1^(+)Arg1^(+)细胞(M2型胶质细胞)数较Sham组增加,而CUR组大鼠视网膜Iba-1^(+)Arg1^(+)细胞数显著高于RIRI组(均为P<0.05)。免疫组织化学染色结果显示,与Sham组相比,RIRI组大鼠视网膜p-JAK2^(+)、p-STAT3^(+)细胞数均较Sham组增多,而CUR组大鼠视网膜p-JAK2^(+)、p-STAT3^(+)细胞数均显著少于RIRI组(均为P<0.05)。Western blot检测结果显示,Sham组大鼠视网膜p-JAK2和p-STAT3蛋白表达水平较低;造模后24 h,RIRI组大鼠视网膜p-JAK2和p-STAT3蛋白Objective To explore the effect of Curcumin(CUR)on the retinal microglia polarization in retinal ischemia reperfusion injury(RIRI)rats and analyze its mechanism via the janus kinase 2(JAK2)/signal transducer and activator of transcription 3(STAT3)pathway,providing the theoretical basis for the clinical application of CUR.Methods Totally 36 healthy male adult Sprague Dawley rats were randomly divided into the Sham group,RIRI group and CUR group,with 12 in each group.The RIRI models were established in the right eye of rats in the RIRI group and CUR group by the high intraocular pressure method.In the Sham group,the needle was only inserted into the anterior chamber of the right eye without increasing intraocular pressure.At 30 min before the modeling,rats in the CUR group were intraperitoneally injected with CUR(100 mg·kg^(-1)),while rats in the RIRI group and Sham group were intraperitoneally injected with an equal dose of physiological saline.At 24 h after modeling,the retinal morphology and the number of retinal ganglion cells(RGC)of rats in each group were observed by hematoxylin-eosin(HE)staining and immunohistochemical staining.Iba-1/CD16 and Iba-1/Arg1 immunofluorescence staining was used to detect the effect of CUR on the polarization of retinal microglia in RIRI rats.Immunohistochemical staining combined with Western blot was used to observe the effect of CUR on the expression of RGC and the molecular expression of JAK2/STAT3 signaling pathway in RIRI rats.Results The HE staining and immunohistochemical staining showed that the retinal cells of rats in the Sham group were neatly arranged,and a large number of Brn-3a^(+)cells(that is,RGC)were found in the ganglion cell layer.Compared with the Sham group,the thickness of the inner retinal layer increased significantly,and the number of RGC decreased significantly in the RIRI group 24 h after mode\|ling;the thickness of the inner retinal layer in the CUR group was significantly thinner than that in the RIRI group,and the number of RGC was significantly high

关 键 词：视网膜缺血-再灌注损伤 小胶质细胞极化 姜黄素 JAK2/STAT3通路 

分 类 号：R774.1[医药卫生—眼科]