白藜芦醇减轻高原低压低氧大鼠心功能损伤的作用  

Resveratrol attenuates cardiac function impairment in plateau hypobaric hypoxia rats

作　　者：徐洪宝 宋晓娜 闫长青 王光瑞 蒲玲玲 王紫瑈 王新兴 陈照立 刘伟丽 XU Hong-bao;SONG Xiao-na;YAN Chang-qing;WANG Guang-rui;PU Ling-ling;WANG Zi-rou;WANG Xin-xing;CHEN Zhao-li;LIU Wei-li(Institute of Environmental and Operational Medicine,Academy of Military Medical Science,Academy of Military Sciences,Tianjin 300050,China)

机构地区：[1]军事科学院军事医学研究院环境医学与作业医学研究所,天津300050

出　　处：《中国应用生理学杂志》2022年第6期644-649,共6页Chinese Journal of Applied Physiology

基　　金：后勤科研计划重点项目(BLJ20J011)。

摘　　要：目的:研究白藜芦醇(RSV)对高原低压低氧大鼠心功能的保护作用及机制。方法:将36只大鼠按照随机数字法分为对照组(Control,C)、低压低氧组(HH)和低压低氧+RSV组(HH+RSV),每组12只。HH组和HH+RSV组大鼠在低压氧舱模拟海拔6000 m,20 h/d进行慢性长期高原低压低氧干预8周,RSV灌喂浓度为400 mg/(kg·d)。大鼠每周检测1次体重、2次摄食量,大鼠处死前采用血细胞分析仪检测各组血常规指标、超声心动图检测各组大鼠心功能指标,采用HE染色评价心肌肥厚情况,采用二氢乙啶(DHE)染色评价心肌组织活性氧水平,采用血清及心肌组织总抗氧化能力(T-AOC)、超氧化物歧化酶活性(SOD)、丙二醛(MDA)含量评价氧化应激情况。结果:与C组相比,HH组大鼠体质量和摄食量显著降低(P<0.05);而与HH组相比,HH+RSV组大鼠体质量及摄食量无显著影响(P>0.05)。血常规结果显示,与C组相比,HH组大鼠红细胞和血红蛋白显著升高(P<0.05),血小板浓度显著降低(P<0.05);与HH组相比,HH+RSV组大鼠红细胞和血红蛋白水平显著降低(P<0.05),血小板浓度显著升高(P<0.05)。与C组相比,HH组心脏系数、心肌纤维直径和厚度显著增大(P<0.05);与HH组相比,HH+RSV组大鼠心脏系数和心肌纤维厚度显著下降(P<0.05)。超声心动图分析显示,与C组相比,HH组大鼠心室壁厚度显著增加(P<0.05),射血分数及心输出量显著降低(P<0.05);与HH组相比,HH+RSV组大鼠心室壁厚度显著降低,心脏功能显著改善(P<0.05)。DHE染色结果显示,与C组相比,HH组大鼠心肌组织活性氧水平显著升高(P<0.05);与HH组相比,HH+RSV组大鼠心肌组织活性氧水平明显恢复(P<0.05)。氧化抗氧化结果显示,与C组相比,HH组大鼠血清及心肌T-AOC、SOD活性显著降低(P<0.05),MDA含量显著升高(P<0.05);与HH组相比,HH+RSV组大鼠血清及心肌T-AOC、SOD活性显著升高(P<0.05),MDA含量显著降低(P<0.05)。结论:长期高原低压低氧暴露导致大�Objective:To study the protective effects of resveratrol(RSV)on cardiac function in rats with high altitude hypobaric hypoxia and its mechanisms.Methods:Thirty-six rats were randomly divided into control group,hypobaric hypoxia group(HH)and hypobaric hypoxia+RSV group(HH+RSV)according to the random number,12 rats in each group.Rats in the HH and HH+RSV groups were subjected to chronic long-term high altitude hypobaric hypoxia intervention for 8 weeks in a hypobaric chamber at a simulated altitude of 6000 m for 20 h/d.The rats of HH+RSV were fed with RSV at a dose of 400 mg/(kg·d).The rats were tested once a week for body weight and twice a week for food intake.Before execution,the rats were tested by blood cell analyzer for routine blood parameters and echocardiogram for cardiac function parameters in each group.The routine blood indexes of each group were measured by blood cell analyzer,the cardiac function indexes of each group were measured by echocardiography,myocardial hypertrophy was evaluated by HE staining,myocardial tissue reactive oxygen levels were evaluated by dihydroethidium(DHE)staining.Oxidative stress was evaluated by serum and myocardial tissue total antioxidant capacity(T-AOC),superoxide dismutase activity(SOD)and malondialdehyde(MDA)content.Results:Compared with the C group,the body mass and food intake of rats were decreased significantly(P<0.05)in HH group,while compared with the C group,RSV had no significant effects on the body mass and food intake of rats in the HH+RSV group(P>0.05).Compared with the C group,the levels of erythrocytes and hemoglobin of rats in the HH group were increased significantly(P<0.05),while the platelet concentration was decreased significantly(P<0.05);compared with the HH group,the erythrocyte and hemoglobin levels were decreased significantly(P<0.05)and platelet concentration was increased significantly(P<0.05)in rats of the HH+RSV group.Compared with the C group,the cardiac coefficient,myocardial fiber diameter and thickness were significantly increased in the

关 键 词：低压低氧 白藜芦醇 心肌肥厚 超声心动图 大鼠 

分 类 号：R361.2[医药卫生—病理学]