姜黄素对慢性酒精成瘾致肝损伤的干预作用  

Effects of curcumin on liver injury induced by chronic alcohol addiction

作　　者：吴一鸣 林雪 苏雅静 薛頔 张晨[1] WU Yi-ming;LIN Xue;SU Ya-jing;XUE Di;ZHANG Chen(College of Education,Ningxia University,Yinchuan 750021;Medical Research Institute of General Hospital of Ningxia Medical University;Ningxia Key Laboratory of Clinical and Pathogenic Microbiology,General Hospital of Ningxia Medical University,Yinchuan 750004;College of Life Science,Ningxia University,Yinchuan 750021,China)

机构地区：[1]宁夏大学教育学院,银川750021 [2]宁夏医科大学总医院医学研究院,银川750004 [3]宁夏医科大学总医院临床病原微生物学宁夏重点实验室,银川750004 [4]宁夏大学生命科学学院,银川750021

出　　处：《中国应用生理学杂志》2022年第6期782-786,共5页Chinese Journal of Applied Physiology

基　　金：宁夏回族自治区重点研发项目(2022BEG03126);宁夏回族自治区重点研发项目(2020BEG03019);宁夏自然科学基金(2021AAC03109);宁夏自然科学基金(2022AAC03029)。

摘　　要：目的:探讨姜黄素(Curc)对慢性酒精成瘾性引起的小鼠肝损伤的干预作用。方法:将30只Balb/c小鼠随机分为正常对照组(Control)、模型组(Model)、低剂量Curc组(5 mg/kg,Curc-L)、中剂量Curc组(10 mg/kg,Curc-M)和高剂量Curc组(15 mg/kg,Curc-H),每组6只。采用20%的白酒制备慢性酒精成瘾性肝损伤模型,Con组每天灌胃2 ml生理盐水;Mod组每天灌胃20%白酒5 ml/kg,Curc治疗组每天用对应浓度的Curc灌胃,每次2 ml,连续35 d。测量小鼠肝脏重量,观察小鼠健康状况。检测小鼠血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸酶(ALP)和肝脏中甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、丙二醛(MDA)、总超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、一氧化氮(NO)浓度。观察苏木精和伊红染色的肝组织病理形态学变化。结果:与对照组相比,模型组小鼠肝脏质量及血清中ALT、AST、ALP、MDA、NO、TC、TG、HDL-C、LDL-C水平显著升高(P<0.05,P<0.01),SOD和GSH-Px活力显著降低(P<0.05,P<0.01),肝细胞有空泡和炎性细胞浸润,肝组织NF-κB和MAPK蛋白表达水平显著上调(P<0.01)。与模型组相比,Curc组的ALT、AST、ALP、MDA、NO、TC、TG、HDL-C、LDL-C的水平显著降低并SOD和GSH-Px活力显著升高(P<0.05,P<0.01)。结论:Curc可能通过调控NF-κB/MAPK信号通路有效地减少肝脏组织损伤。Objective:To investigate the intervention effects of curcumin(Curc)on liver injury induced by chronic alcohol addiction in mice.Methods:Thirty Balb/c mice were randomly divided into normal control group(Control),model group(Model),low-dose Curc group(5 mg/kg,Curc-L),medium dose Curc group(10 mg/kg,Curc-M)and high-dose Curc group(15 mg/kg,Curc-H),with 6 mice in each group.The chronic alcohol addiction liver injury model was prepared with 20%liquor.The mice in control group were given 2 ml of normal saline every day.The mice in model group were given 5 ml/kg of 20%liquor every day,and the mice in Curc treatment group were treated with Curc at the doses of 5,10,15 mg/kg in 2 ml saline every day for 35 days.The weight of liver was measured and the health status of mice was observed.Serum ALT,AST,ALP and liver TG,TC,HDL-C,LDL-C,MDA,SOD,GSH-Px and NO were measured.The pathological changes of liver tissues stained with hematoxylin and eosin were observed.Results:Compared with the control group,the liver mass and serum levels of ALT,AST,ALP,MDA,NO,TC,TG,HDL-C,LDL-C in the model group were increased significantly(P<0.05,P<0.01),the activities of SOD and GSH-Px were decreased significantly(P<0.05,P<0.01),the liver cells were vacuolated and infiltrated with inflammatory cells,and the expression levels of NF-κB and MAPK protein in liver tissues were increased significantly(P<0.01).Compared with the model group,the levels of ALT,AST,ALP,MDA,NO,TC,TG,HDL-C,LDL-C in Curc group were decreased significantly nd the activities of SOD and GSH-Px were increased significantly(P<0.05,P<0.01).Conclusion:Curc can effectively reduce liver tissue damage by regulating NF-κB/MAPK signal pathway.

关 键 词：姜黄素 慢性酒精成瘾 酒精性肝损伤 氧化应激 BALB/C小鼠 

分 类 号：R575.2[医药卫生—消化系统]