miR-4783-3p在肝癌组织中的表达及对肝癌细胞增殖和迁移的影响  

Expression of miR-4783-3p in hepatocellular carcinoma tissue and its effect on the proliferation and migration of hepatocellular carcinoma cells

作　　者：彭强 何承峻[1] 王健宇 杨波 刘希 Peng Qiang;He Chengjun;Wang Jianyu;Yang Bo;Liu Xi(Department of Hepatobiliary and Pancreatic Surgery,the First People's Hospital of Ziyang City,Ziyang 641300 China;Department of Hepatobiliary and Pancreatic Surgery,the Second Afiliated Hospital of Zhejiang University,Hangzhou 310052,China)

机构地区：[1]资阳市第一人民医院肝胆胰外科,资阳641300 [2]浙江大学附属第二医院肝胆胰外科,杭州310052

出　　处：《国际外科学杂志》2023年第4期270-275,F0004,共7页International Journal of Surgery

基　　金：国家自然科学基金(81773753)。

摘　　要：目的研究miR-4783-3p在肝癌组织中的表达及对肝癌Huh-7细胞增殖及迁移产生的影响。方法采用cBioPortal数据库分析肝癌组织和癌旁组织中miR-4783-3p的表达,严格按照Lipofectamine^(TM)2000转染试剂盒说明书,分别将miR-4783-3p过表达模拟物或过表达对照模拟物转染至Huh-7细胞,即过表达组和对照组。通过CCK-8实验分析Huh-7细胞增殖情况,细胞划痕法分析Huh-7细胞迁移情况。双荧光素酶报告基因法检测miR-4783-3p与胰岛素样生长因子结合蛋白2(IGFBP2)mRNA的靶向关系。实时荧光定量聚合酶链反应(RT-qPCR)检测IGFBP2 mRNA的表达。Western-blotting检测IGFBP2蛋白和EGFR-STAT3分子通路蛋白的表达。正态分布的计量资料以均数±标准差(x±s)表示,组间比较采用t检验或单因素方差分析。结果肝癌组织中miR-4783-3p的表达明显低于癌旁组织(P<0.01)。与对照组比较,过表达组Huh-7细胞增殖能力明显降低(P<0.05)。对照组和过表达组中Huh-7细胞划痕愈合率分别为(67.71±9.04)%和(29.58±10.51)%,过表达组划痕愈合率明显降低(P<0.01)。miR-4783-3p靶向结合IGFBP2 mRNA(P<0.01)。对照组和过表达组中IGFBP2 mRNA表达分别为5.76±1.44和0.99±0.47,miR-4783-3p负调控IGFBP2 mRNA表达(P<0.01)。与对照组比较,过表达组IGFBP2蛋白和EGFR-STAT3分子通路蛋白表达均降低。结论miR-4783-3p在肝癌中低表达,其能通过诱导IGFBP2基因低表达,减弱肝癌Huh-7细胞的增殖及侵袭能力。Objective To study the expression of microRNA(miRNA)-4783-3p in liver cancer tissue and its effect on the proliferation and migration of liver cancer Huh-7 cells.Methods The cBioPortal database was used to analyze the expression of miR-4783-3p in liver cancer tissues and adjacent tissues.In strict accordance with the instructions of Lipofectamine^(TM)2000 transfection kit,miR-4783-3p overexpression mimics or overexpression control mimics were transfected into Huh-7 cells respectively,namely overexpression group and control group.The proliferation of Huh-7 cells was analyzed by CCK-8 assay,and the migration of Huh-7 cells was analyzed by cell scratch method.The targeting relationship between miR-4783-3p and insulin-like growth factor binding protein 2(IGFBP2)mRNA was detected by dual-luciferase reporter gene assay.RT-qPCR was used to detect the expression of IGFBP2 mRNA.Western-blotting was used to detect the expression of IGFBP2 protein and EGFR-STAT3 molecular pathway proteins.Results The expression of miR-4783-3p in liver cancer tissues was significantly lower than that in adjacent tissues(P<0.01).Compared with the control group,the proliferation ability of Huh-7 cells in the overexpression group was significantly decreased(P<0.05).The scratch healing rates of Huh-7 cells in the control group and the overexpression group were(67.71±9.04)%and(29.58±10.51)%,respectively,and the scratch healing rate in the overexpression group was significantly lower(P<0.01).miR-4783-3p targeted and bound to IGFBP2 mRNA(P<0.01).The expression of IGFBP2 mRNA in the control and overexpression groups was 5.76±1.44 and 0.99±0.47,respectively,and miR-4783-3p negatively regulated the expression of IGFBP2 mRNA(P<0.01).Compared with the control group,the expressions of IGFBP2 protein and EGFR-STAT3 molecular pathway protein were decreased in the overexpression group.Conclusions miR-4783-3p is lowly expressed in liver cancer tissues.miR-4783-3p can attenuate the proliferation and invasion ability of liver cancer Huh-7 cells by inducing

关 键 词：肝肿瘤 细胞增殖 细胞迁移分析 miR-4783-3p 胰岛素样生长因子结合蛋白2 

分 类 号：R735.7[医药卫生—肿瘤]