外周血hsa_circ_0105035表达水平与急性缺血性脑卒中的相关性  

Correlation between the expression level of hsa_circ_0105035 in peripheral blood and acute ischemic stroke

作　　者：梁雪莹 赵直 龙建雄[1] 朱路路 杨佳磊 苏莉[1] LIANG Xueying;ZHAO Zhi;LONG Jianxiong;ZHU Lulu;YANG Jialei;SU Li(School of Public Health,Guangxi Medical University,Nanning 530021,Guangxi,China)

机构地区：[1]广西医科大学公共卫生学院,广西南宁市530021

出　　处：《微创医学》2023年第2期151-158,共8页Journal of Minimally Invasive Medicine

基　　金：国家自然科学基金(编号:81874395);广西自然科学基金(编号:2018GXNSFAA281224、2017GXNSFDA198013)。

摘　　要：目的探讨外周血hsa_circ_0105035表达水平与急性缺血性脑卒中(AIS)发生的关系,以及与脑梗死体积和临床检测指标的关联。方法纳入AIS患者310例为病例组,另选择同期年龄与病例组相匹配的无脑卒中、冠心病等心脑血管疾病史的社区人群310例为对照组,采用实时荧光定量PCR(qRT-PCR)测定所有研究对象外周血hsa_circ_0105035的表达水平,以Sanger测序和线性消化酶(RNase R)实验验证其环状结构,以核质分离实验明确其亚细胞定位。通过Image J软件计算得到AIS患者的脑梗死体积。结果hsa_circ_0105035主要分布于细胞质。病例组患者的外周血hsa_circ_0105035表达水平低于对照组,差异有统计学意义(P<0.05)。AIS患者外周血hsa_circ_0105035的表达水平与脑梗死体积呈负相关(rs=-0.127,P=0.045)。hsa_circ_0105035低表达组AIS患者的血小板体积分布宽度、乳酸脱氢酶含量高于hsa_circ_0105035高表达组,差异均有统计学意义(均P<0.05)。结论外周血hsa_circ_0105035表达可能与AIS的发生相关,并且与脑梗死体积大小有关。Objective To investigate the correlation between the expression level of hsa_circ_0105035 in peripheral blood and the occurrence of acute ischemic stroke(AIS),as well as its correlation with cerebral infarction volume and clinical detection indicators.Methods A total of 310 AIS patients were enrolled as the case group,while another 310 age-matched community people without a history of cardiovascular and cerebrovascular diseases such as stroke or coronary heart disease during the same period was selected as the control group.The expression levels of hsa_circ_0105035 in peripheral blood of all research subjects were detected by quantitative real-time PCR(qRT-PCR),its circular structure was verified by Sanger sequencing and linear digestive enzyme(RNase-R)experiment,and its subcellular location was clarified by nucleocytoplasmic fraction experiment.The cerebral infarction volume of AIS patients was calculated by the Image J software.Results Hsa_circ_0105035 was mainly distributed in cytoplasm.The expression level of hsa_circ_0105035 in peripheral blood of the case group was lower than that of the control group,with statistically significant difference(P<0.05).The expression level of hsa_circ_0105035 in peripheral blood of AIS patients was negatively correlated with cerebral infarction volume(r s=-0.127,P=0.045).The platelet volume distribution width and lactate dehydrogenase content of AIS patients in the hsa_circ_0105035 low expression group were larger/higher than those in the hsa_circ_0105035 high expression group,with statistically significant differences(all P<0.05).Conclusion The expression of hsa_circ_0105035 in peripheral blood may be correlated with the occurrence of AIS and the cerebral infarction volume.

关 键 词：急性缺血性脑卒中 hsa_circ_0105035 环状RNA 脑梗死体积 

分 类 号：R743.3[医药卫生—神经病学与精神病学]