Human pluripotent stem cell-derived extracellular vesicles:From now to the future  

作　　者：Bruno Moises de Matos Marco Augusto Stimamiglio Alejandro Correa Anny Waloski Robert 

机构地区：[1]Stem Cells Basic Biology Laboratory,Carlos Chagas Institute,Curitiba 81350010,Paraná,Brazil

出　　处：《World Journal of Stem Cells》2023年第5期453-465,共13页世界干细胞杂志（英文版）（电子版）

摘　　要：Extracellular vesicles(EVs)are nanometric particles that enclose cell-derived bioactive molecules in a lipid bilayer and serve as intercellular communication tools.Accordingly,in various biological contexts,EVs are reported to engage in immune modulation,senescence,and cell proliferation and differentiation.Therefore,EVs could be key elements for potential off-the-shelf cell-free therapy.Little has been studied regarding EVs derived from human pluripotent stem cells(hPSC-EVs),even though hPSCs offer good opportunities for induction of tissue regeneration and unlimited proliferative ability.In this review article,we provide an overview of studies using hPSC-EVs,focusing on identifying the conditions in which the cells are cultivated for the isolation of EVs,how they are characterized,and applications already demonstrated.The topics reported in this article highlight the incipient status of the studies in the field and the significance of hPSC-EVs’prospective applications as PSC-derived cell-free therapy products.

关 键 词：Pluripotent stem cells Extracellular vesicles EXOSOME Cell-free therapy 

分 类 号：R318[医药卫生—生物医学工程]