miR-574-5p和miR-144-5p在腹主动脉瘤患者外周血中的表达及其与腔内血栓厚度的关系  

The Expression of miR-574-5p and miR-144-5p in Peripheral Blood of Patients with Abdominal Aortic Aneurysm and Their Relationship with Intracavitary Thrombus Thickness

作　　者：徐鹏[1] 单金涛 孙利坤 XU Peng;SHAN Jintao;SUN Likun(The First Affiliated Hospital of Zhengzhou University,Zhengzhou,450000)

机构地区：[1]郑州大学第一附属医院,450000

出　　处：《实用癌症杂志》2023年第6期910-913,共4页The Practical Journal of Cancer

摘　　要：目的分析腹主动脉瘤(AAA)患者外周血内微小RNA-574-5p(miR-574-5p)、微小RNA-144-5p(miR-144-5p)的表达及其与腔内血栓(ILT)厚度的关系。方法选取84例AAA患者纳入观察组,另选取同期行体检的75例健康人群纳入对照组,采集两组外周静脉血,检测对比其miR-574-5p、miR-144-5p差异;另外,对观察组患者行彩色多普勒超声检测,连续检测3次,取其平均值,依据其ILT厚度的不同将所有患者分为薄ILT组(n=48,ILT厚度≤平均值)与厚ILT组(n=36,ILT厚度>平均值)两个亚组;统计对比两组的miR-574-5p、miR-144-5p水平;另外,绘制受试者工作曲线(ROC),分析miR-574-5p、miR-144-5p两者联合预测AAA患者ILT厚度的效能。结果观察组miR-574-5p高于对照组,miR-144-5p低于对照组,差异有统计学意义(P<0.05);厚ILT组miR-574-5p高于薄ILT组,miR-144-5p低于薄ILT组,差异有统计学意义(P<0.05);ROC结果显示:miR-574-5p、miR-144-5p两者联合预测AAA患者ILT厚度的曲线下面积(AUC)高于两者单独预测。结论AAA患者外周血内的miR-574-5p、miR-144-5p呈异常表达,而miR-574-5p、miR-144-5p与患者的ILT厚度密切相关,miR-574-5p越高,miR-144-5p越低,则AAA患者的ILT厚度越大,两者联合检测可有效反映AAA患者的ILT厚度,临床应用价值较高,可进行大力的推行。Objective To analyze the expression of microRNA-574-5p(miR-574-5p)and microRNA-144-5p(miR-144-5p)in peripheral blood of patients with abdominal aortic aneurysm(AAA)and their relationship with the thickness of intracavitary thrombus(ILT).Methods 84 AAA patients were selected into the observation group,and 75 healthy patients were selected into the control group.Peripheral venous blood was collected from the 2 groups,and their miR-574-5p and miR-144-5p differences were detected and compared;In addition,the observation group patients were subjected to color Doppler ultrasound testing 3 consecutive times,and their average values were taken.Based on their different ILT thicknesses,all patients were divided into 2 subgroups:the thin ILT group(n=48,ILT thickness≤average)and the thick ILT group(n=36,ILT thickness>average);Compare the levels of miR-574-5p and miR-144-5p between the 2 groups;In addition,draw the receiver operating curve(ROC)and analyze the efficacy of combining miR-574-5p and miR-144-5p in predicting ILT thickness in AAA patients.Results The results showed that the miR-574-5p in the observation group was higher than that in the control group,while the miR-144-5p was lower than that in the control group,with a statistically significant difference(P<0.05);The miR-574-5p in the thick ILT group was higher than that in the thin ILT group,and the miR-144-5p was lower than that in the thin ILT group,with a statistically significant difference(P<0.05);The ROC results showed that the combined prediction of miR-574-5p and miR-144-5p for the area under the curve(AUC)of ILT thickness in AAA patients was higher than that predicted solely by both.Conclusion The expression of miR-574-5p and miR-144-5p in the peripheral blood of AAA patients is abnormal,and miR-574-5p and miR-144-5p are closely related to the ILT thickness of the patients.The higher the miR-574-5p,the lower the miR-144-5p,the larger the ILT thickness of AAA patients.Combined detection of the 2 can effectively reflect the ILT thickness of AAA patients,wi

关 键 词：腹主动脉瘤 腔内血栓厚度 外周血内微小RNA-574-5p 微小RNA-144-5p 

分 类 号：R732.21[医药卫生—肿瘤]