超声造影联合血清miR-122-5p、miR-133a-3p水平对肝细胞癌的诊断价值  

The Diagnostic Value of Ultrasound Contrast Combined with Serum miR-122-5p and miR-133a-3p Levels in Hepatocellular Carcinoma

作　　者：王晓静 赵晓娟[1] 任晓转 WANG Xiaojing;ZHAO Xiaojuan;REN Xiaozhuan(Henan People's Hospital,Zhengzhou,450000)

机构地区：[1]河南省人民医院,450000

出　　处：《实用癌症杂志》2023年第6期964-968,共5页The Practical Journal of Cancer

基　　金：河南省医学科技攻关计划项目(编号:LHGJ20220058)。

摘　　要：目的探究超声造影(CEUS)联合血清miR-122-5p、miR-133a-3p水平对肝细胞癌(HCC)的诊断价值。方法选取45例HCC患者及45例慢性乙型肝炎患者作为研究对象。采集患者血液标本,分离血清,采用荧光定量聚合酶链反应(RT-qPCR)检测患者血清miR-122-5p、miR-133a-3p表达量。同时为患者进行CEUS,评估其诊断价值。另外绘制受试者工作曲线(ROC)、进行COX多因素分析评估CEUS联合血清miR-122-5p、miR-133a-3p水平对肝细胞癌的诊断价值。结果HCC组始消时间、持续时间、达峰时间及始增时间均低于对照组(P<0.05);绘制CEUS各项指标诊断HCC的ROC曲线显示,单独检测时始增时间的敏感度最高,始消时间、持续时间的特异度最高,联合检测的AUC为0.978,特异度及敏感度均处于较高水平。HCC组血清miR-122-5p、miR-133a-3p表达水平均明显低于对照组;绘制血清miR-122-5p、miR-133a-3p水平诊断HCC的ROC曲线,单独检测时,miR-133a-3p的敏感度最高,miR-122-5p的特异度最高,两者联合检测具有较高的敏感度及特异度。CEUS联合血清miR-122-5p、miR-133a-3p水平诊断HCC的ROC曲线显示,AUC为0.999,截断值为0.712,敏感度、特异度分别为97.78%,99.99%,约登指数为0.9778,95%CI为0.958~1.000。行多因素COX分析显示,始消时间、达峰时间、始增时间、血清miR-122-5p、miR-133a-3p均为影响HCC诊断的因素(P<0.05)。结论CEUS联合血清miR-122-5p、miR-133a-3p水平对肝细胞癌具有较高的诊断价值。Objective To investigate the diagnostic value of contrast ultrasonography(CEUS)combined with serum miR-122-5p and miR-133a-3p levels in the diagnosis of hepatocellular carcinoma(HCC).Methods 45 patients with HCC and 45 patients with chronic hepatitis B who received treatment were enrolled as the study subjects,and the general data of the patients were collected.Blood samples were collected,serum was isolated,and the expression of serum miR-122-5p and miR-133a-3p was detected by fluorescence quantitative polymerase chain reaction(RT-qPCR).CEUS was also performed for patients to assess their diagnostic value.In addition,the receiver working curve(ROC)was plotted and COX multivariate analysis was performed to evaluate the diagnostic value of CEUS combined with serum miR-122-5p and miR-133a-3p levels for hepatocellular carcinoma.Results The initiation time,duration,peak time and initial increase time of HCC group were lower than those of the control group(P<0.05).The ROC curve of CEUS indexes for the diagnosis of HCC showed that the sensitivity of the initial increase time was the highest,the specificity of the elimination time and duration were the highest when detected alone,and the AUC of the combined detection was 0.978,and the specificity and sensitivity were at a high level.The serum expression levels of miR-122-5p and miR-133a-3p in the HCC group were significantly lower than those in the control group.The ROC curves of serum miR-122-5p and miR-133a-3p levels for HCC were plotted,and the sensitivity of miR-133a-3p was the highest and the specificity of miR-122-5p was the highest when detected alone,and the combined detection of the 2 had high sensitivity and specificity.The ROC curves of CEUS combined with serum miR-122-5p and miR-133a-3p levels for HCC showed that the AUC was 0.999,the cut-off value was 0.712,the sensitivity and specificity were 97.78% and 99.99%,the Yoden index was 0.9778,and the 95%CI was 0.958~1.000.Multivariate COX analysis showed that the time of initiation and disappearance,peak time,ti

关 键 词：肝细胞癌 超声造影 miR-133a-3p miR-122-5p 诊断 

分 类 号：R735.7[医药卫生—肿瘤]