hsacircRNA0002141靶向miR-217影响口腔癌进展的分子机制研究  

Molecular mechanism of hsa_circRNA_0002141 targeting miR-217 affecting progression of oral cancer

作　　者：宿伟鹏[1] 赵化荣[1] 李晨曦[2,3] 刘攀[1] 张洋[1] 龚忠诚[2,3] SU Weipeng;ZHAO Huarong;LI Chenxi;LIU Pan;ZHANG Yang;GONG Zhongcheng(Center of Oncology,the First Affiliated Hospital of Xinjiang Medical University/Affiliated Stomatological Hospital,Urumqi 830054,China;Department of Oral and Maxillofacial Oncology Surgery,the First Affiliated Hospital of Xinjiang Medical University/Affiliated Stomatological Hospital,Urumqi 830054,China;the First Affiliated Hospital of Xinjiang Medical University/Affiliated Stomatological Hospital,Stomatological Research Institute of Xinjiang Uygur Autonomous Region,Urumqi 830054,China)

机构地区：[1]新疆医科大学第一附属医院肿瘤中心,乌鲁木齐830054 [2]新疆医科大学第一附属医院(附属口腔医院)颌面肿瘤外科,乌鲁木齐830054 [3]新疆医科大学第一附属医院新疆维吾尔自治区口腔医学研究所,乌鲁木齐830054

出　　处：《新疆医科大学学报》2023年第5期588-595,共8页Journal of Xinjiang Medical University

基　　金：国家自然科学基金项目(82160189);口腔颌面发育与再生湖北省重点实验室开放课题项目(2022kqhm008);新疆维吾尔自治区天山创新团队项目(2021D14001)。

摘　　要：目的探讨人环状RNA(hsa_circRNA)0002141在口腔癌细胞生物学进展中的作用及其机制。方法应用实时荧光定量聚合酶链式反应(qRT-PCR)检测人口腔鳞状细胞癌HSC3、SAS、SCC15、FaDu细胞与人正常口腔角质形成HNOK细胞中hsa_circ_0002141、miR-217的表达差异,生物信息学软件预测hsa_circ_0002141与miR-217的靶向结合位点,双荧光素酶报告基因检测细胞荧光素酶活性;将si_NC组、si_circ_0002141组、pc_NC组、pc_circ_0002141组、pc_circ_0002141+miR-NC组、pc_circ_0002141+miR-217 mimic组均根据脂质体法转染口腔癌细胞SAS,以正常培养的SAS细胞作为对照组,CCK-8法检测各组细胞增殖活性,EdU染色检测各组细胞增殖水平,Transwell实验测定各组细胞迁移与侵袭情况。结果与人正常口腔角质形成HNOK细胞比较,在人口腔鳞状细胞癌HSC3、SAS、SCC15、FaDu细胞中,hsa_circ_0002141表达显著上调,而miR-217表达显著下调(P<0.05)。hsa_circ_0002141与miR-217之间存在互补靶向结合位点,miR-217靶向负调控hsa_circ_0002141表达(P<0.05)。与对照组比较,si_circ_0002141组SAS细胞增殖活性下降,EdU阳性率降低,细胞迁移与细胞侵袭数目均减少(P<0.05);而pc_circ_0002141组细胞增殖活性上升,EdU阳性率升高,细胞迁移与细胞侵袭数目均增加(P<0.05)。与pc_circ_0002141组比较,pc_circ_0002141+miR-217 mimic组细胞增殖活性下降,EdU阳性率降低,细胞迁移与细胞侵袭数目均减少(P<0.05)。结论hsa_circ_0002141可调控SAS细胞的增殖、迁移及侵袭的生物学行为,该机制可能与其靶向miR-217有关。Objective To explore the role and mechanism of human circular RNA(hsa_circRNA)0002141 in the biological progression of oral cancer.Methods The expression levels of hsa_circ_0002141 and miR-217 in human oral squamous cell carcinoma HSC3,SAS,SCC15,FaDu cells and human normal oral keratinocyte HNOK cells were detected by real-time quantitative polymerase chain reaction(qRT-PCR),the targeted binding sites of hsa_circ_0002141 and miR-217 were predicted by bioinformatics software,and cellular luciferase activity was detected by the dual luciferase reporter gene method.The oral cancer SAS cells were divided into si_NC group,si_circ_0002141 group,pc_NC group,pc_circ_0002141 group,pc_circ_0002141+miR-NC group and pc_circ_0002141+miR-217 mimic group,these groups were all transfected according to the liposome method,the normal cultured SAS cells were used as control group,CCK-8 method was used to detect the proliferation activity of the cells in each group.EdU staining was used to detect the level of cell proliferation in each group.Transwell assay was used to determine the migration and invasion of the cells in each group.Results Compared with human normal oral keratinocytes HNOK,hsa_circ_0002141 was significantly up-regulated in human oral squamous cell carcinoma HSC3,SAS,SCC15,and FaDu cells,while miR-217 was significantly down-regulated(P<0.05).There is a complementary targeting binding site between hsa_circ_0002141 and miR-217,and miR-217 negatively regulates the expression of hsa_circ_0002141(P<0.05).Compared with control group,the proliferation activity of SAS cells in si_circ_0002141 group were decreased,the EdU positive rate were decreased,the number of cell migration and cell invasion were decreased(P<0.05).In pc_circ_0002141 group,cell proliferation activity,EdU positive rate,cell migration and cell invasion number were increased(P<0.05).In addition,compared with pc_circ_0002141 group,the cell proliferation activity of pc_circ_0002141+miR-217 mimic group was decreased again,the EdU positive rate also decreased,an

关 键 词：口腔癌 hsa_circRNA_0002141 miR-217 增殖 迁移 侵袭 

分 类 号：R739.8[医药卫生—肿瘤]