WR-2721与香菇多糖、细胞因子在抗辐射效应中的联合作用  

Protective effects of WR-2721 combined with lentinan and cytokines against radiation damage

作　　者：刘玉明 江叔奇[2] 蒋定文 何颖 李珂娴 侯登勇 LIU Yuming;JIANG Shuqi;JIANG Dingwen;HE Ying;LI Kexian;HOU Dengyong(Naval Characteristic Medical Center,Shanghai 200433 China;Zhejiang Chinese Medicine University,Hangzhou 310053 China)

机构地区：[1]海军特色医学中心,上海200433 [2]浙江中医药大学,浙江杭州310053

出　　处：《中国辐射卫生》2023年第2期156-160,166,共6页Chinese Journal of Radiological Health

基　　金：国家科技重大专项重大新药创制项目(2008ZXJ09004-027)。

摘　　要：目的 研究WR-2721与香菇多糖、细胞因子联合应用对辐射损伤小鼠的协同保护作用,为急性放射损伤的临床治疗提供一种新的治疗方案。方法 取70只ICR小鼠,分为7组,分别为对照组、模型组、WR-2721组、香菇多糖&细胞因子组、WR-2721&香菇多糖组、WR-2721&细胞因子组、WR-2721&香菇多糖&细胞因子组,除对照组外,其他组用60Co-γ射线一次照射,剂量率为0.8 Gy·min^(-1),累积剂量为5.0 Gy。照后14 d脱臼处死小鼠,测定小鼠脾脏指数、胸腺指数、血清超氧化物歧化酶(SOD)、活性及丙二醛(MDA)、白介素-11(IL-11)、TNF-α含量。结果 WR-2721与香菇多糖、细胞因子联合用药组的小鼠脾脏指数为7.33±2.84、胸腺指数为1.70±0.30、血清SOD为114.0±8.3、MDA为7.33±1.16、IL-11含量为155.8±49.4、TNF-α的含量为174.0±37.8。与模型组相比,除脾脏指数外,其余各指标均具有显著意义(P<0.05或P<0.01),因此联合用药组具有促进辐射损伤恢复的作用。结论 WR-2721与香菇多糖、细胞因子联合应用具有较好的协同保护作用,是急性放射损伤治疗的一种较好的用药方案。Objective To investigate the synergistic protective effects of WR-2721 combined with lentinan and cytokines against radiation damage in mice,and to provide a new treatment for acute radiation injury.Methods Seventy Institute of Cancer Research mice were divided into seven groups:a control group,a model group,WR-2721 group,Lentinan&cytokine group,WR-2721&Lentinan group,WR-2721&cytokine group and WR-2721&Lentinan&cytokine group.All groups except the control group were irradiated with ^(60)Coγ-rays at a dose rate of 0.8 Gy/min and a cumulative dose of 5.0 Gy.The mice were sacrificed by cervical dislocation 14 d after irradiation to measure their spleen index,thymus index,and serum levels of superoxide dismutase(SOD),malondialdehyde(MDA),interleukin-11(IL-11),and tumor necrosis factor-alpha(TNF-α).Results For the mice treated with WR-2721,lentinan,and cytokines,the spleen index was 7.33±2.84,the thymus index was 1.70±0.30,the serum SOD level was 114.0±8.3,the MDA level was 7.33±1.16,the IL-11 level was 155.8±49.4,and the TNF-αlevel was 174.0±37.8.All these indicators except the spleen index in the combination group significantly differed from those of the model group(P<0.05 or 0.01),indicating the combined treatment promoted recovery from radiation damage.Conclusion WR-2721 combined with lentinan and cytokines has significant synergistic protective effects,which is a promising treatment for acute radiation injury.

关 键 词：香菇多糖 协同作用 WR-2721 细胞因子 电离辐射 

分 类 号：R963[医药卫生—微生物与生化药学]