La掺杂ZnO量子点合成及农药残留检测实验设计  

Experimental Design for Synthesis of La-doped ZnO Quantum Dots and Its Application in Pesticide Residues Assay

作　　者：刘晓娟[1] 王钰莹 宋吉英[1] LIU Xiaojuan;WANG Yuying;SONG Jiying(College of Chemistry and Pharmaceutical Sciences,Qingdao Agricultural University,Qingdao 266109,Shandong,China)

机构地区：[1]青岛农业大学化学与药学院,山东青岛266109

出　　处：《实验室研究与探索》2023年第3期5-8,39,共5页Research and Exploration In Laboratory

基　　金：国家自然科学基金面上项目(21775083);山东省高等学校青年创新团队人才引育项目;2021年度青岛农业大学实验技术研究项目(SYJS202111)。

摘　　要：针对食品安全领域中农药残留检测的重大需求,采用简易溶液法合成了La掺杂ZnO量子点作为荧光传感元件,建立了农药残留的开信号荧光检测新方法。该方法利用有机磷农药对乙酰胆碱酯酶(AChE)活性的抑制作用和AChE催化反应生成的乙酸对ZnO量子点的淬灭作用,将有机磷农药的识别转换成荧光信号输出,并对La掺杂ZnO量子点进行了表征及荧光光谱检测。结果表明:相较于纯ZnO量子点,La掺杂能显著提高ZnO量子点的荧光发射强度;当没有农药时,AChE和底物的反应溶液能够迅速淬灭La掺杂ZnO量子点的荧光;而当农药存在时,量子点的荧光基本保持不变。整个实验过程操作简单、反应快速、对以草甘膦为代表的有机磷农药具有很好的分析性能,能够实现农药残留的荧光开信号检测,可应用到食品安全领域。There is a great demand of pesticide residue detection in the field of food safety.Herein,La-doped ZnO quantum dots are fabricated through a facile solution process,which is then utilized as a sensing element to construct a new fluorescent sensing approach for pesticide detection in a“signal-on”mode.The proposed method relies on the inhibition effect of the pesticides on acetylcholinesterase(AChE)activity and the quenching effect of AChE-catalyzed production of acetic acid on the La-doped ZnO quantum dots,which transfers the recognition of pesticide to the output of fluorescence signal.The fabricated La-doped ZnO quantum dots are first characterized and the results show that,compared with the pure ZnO quantum dots,the dope of La remarkably increases the fluorescence emission intensity of the ZnO quantum dots.Additionally,the fluorescence of La-doped ZnO quantum dots is quickly quenched in the presence of the reaction solution of AChE and its substrates,while the fluorescence of the quantum dots keept almost unchanged in the presence of pesticide.The whole experiment procedure possesses the advantages of simple operation and rapid reaction.Using glyphosate as a model organophosphorus pesticide,the present method also exhibits a good analytical performance for pesticide assay.It shows that it can realize the detection of pesticide residues in a“signal-on”mode,which can be applied in the field of food safety.

关 键 词：ZnO量子点 农药残留检测 酶抑制法 

分 类 号：G642.423[文化科学—高等教育学]