三阴性乳腺癌中MIF与D-DT的表达及其临床意义  

Expression and clinical significance of MIF and D-DT in triple negative breast cancer

作　　者：吴燕 陈硕 龙琼先[1] 文彬[3] 黄一凡[3] WU Yan;CHEN Shuo;LONG Qiong-xian;WEN Bin;HUANG Yi-fan(Department of Pathology,Nanchong Central Hospital,the second Clinical College of North Sichuan Medical College;Department of Orthopedics,Nanchong Hospital of Traditional Chinese Medicine;Department of Pathology,North Sichuan Medical College,Nanchong 637000,Sichuan,China)

机构地区：[1]川北医学院第二临床医学院·南充市中心医院病理科 [2]南充市中医医院骨科 [3]川北医学院病理学教研室,四川南充637000

出　　处：《川北医学院学报》2023年第5期595-599,共5页Journal of North Sichuan Medical College

基　　金：四川省卫生健康委员会科研项目(20PJ297)。

摘　　要：目的:探讨MIF、D-DT在三阴性乳腺癌(TNBC)中的表达及其临床意义。方法:收集TNBC存档石蜡组织块99例作为实验组,随机选取同期乳腺纤维腺瘤组织选取20例、TNBC相应癌旁组织99例作为对照。应用免疫组化法检测并比较各组macrophage migration inhibitory factor(MIF)、D-dopachrome tautomerase(D-DT)的表达水平,分析两者表达的相关性及其与临床病理特征、患者生存率的关系。结果:MIF在TNBC组的阳性表达率为71.7%,高于癌旁组织组的11.1%及纤维腺瘤组的10.0%;D-DT在TNBC组的阳性表达率为74.7%,高于癌旁组织组的13.1%及纤维腺瘤组的15.0%,差异均有统计学意义(P<0.05)。MIF在TNBC中的表达与年龄有关(P<0.05);D-DT在TNBC中的表达与组织学分级有关(P<0.05)。MIF与D-DT的表达在TNBC中呈正相关(r=0.254,P=0.011)。MIF阳性患者的生存率为63.6%,阴性患者的生存率为92.9%;D-DT阳性患者的生存率为65.7%,阴性患者的生存率为91.7%;有淋巴结转移患者的生存率为56.5%,无淋巴结转移患者的生存率为87.5%。Kaplan-Meier生存曲线显示,TNBC中MIF、D-DT的表达及淋巴结转移与患者生存率具有相关性(P<0.05)。结论:MIF、D-DT可能与TNBC患者的预后相关。联合检测MIF、D-DT可能为TNBC患者的预后判断及个体化治疗提供新指标、新思路。Objective:To investigate the expression of MIF and D-DT in triple-negative breast cancer(TNBC)and its clinical significance.Methods:99 cases of triple negative breast cancer(TNBC)archived paraffin tissue blocks were collected as the experimental group.20 cases of fibroadenoma of breast and 99 cases of paracancerous tissues were randomly selected as contrast.Immunohistochemical method was used to detect the expression of macrophage migration inhibitory factor(MIF)and D-dopachrome tautomerase(D-DT)The correlation between the expression of the two proteins and their relationship with clinicopathological features and survival rate were analyzed.Results:The positive expression rate of MIF in TNBC group was 71.7%,which was higher than that in paracancerous tissue group(11.1%)and fibroadenoma group(10.0%).The positive expression rate of D-DT in TNBC group was 74.7%,which was higher than that in paracancerous tissue group(13.1%)and fibroadenoma group(15.0%).The differences were statistically significant(P<0.05).The expression of MIF in TNBC was related to age(P<0.05),the expression of D-DT in TNBC was related to histological grading(P<0.05).There was a positive correlation between the expression of MIF and D-DT in TNBC(r=0.254,P=0.011).The survival rate of MIF positive patients was 63.6%,and that of MIF negative patients was 92.9%.The survival rate of D-DT positive patients was 65.7%,and that of D-DT negative patients was 91.7%.The survival rate was 56.5%for patients with lymph node metastasis and 87.5%for patients without lymph node metastasis.Kaplan-Meier survival curve showed that MIF,D-DT and lymph node metastasis were related to the survival rate of TNBC patients(P<0.05).Conclusion:MIF and D-DT may be related to the prognosis of TNBC patients.The combined detection of MIF and D-DT may provide new targets and new ideas for the prognosis and individualized treatment of TNBC patients.

关 键 词：三阴性乳腺癌 巨噬细胞移动抑制因子 D-多巴色素互变异构酶 免疫组化 

分 类 号：R737.9[医药卫生—肿瘤]