p150glued缺失小鼠的脊髓运动神经元中NMDA受体增多  

Loss-of-function of p150glued increases NMDA receptor levels in spinal motor neurons of mice

作　　者：郑佳音 杨璇 于佳[1] Zheng Jiayin;Yang Xuan;Yu Jia(Institute of Geriatrics and Rehabilitation,Bejing Geriatric Hospital,Beijing 100095,China)

机构地区：[1]北京老年医院老年医学基础研究中心,北京100095

出　　处：《脑与神经疾病杂志》2023年第5期269-273,共5页Journal of Brain and Nervous Diseases

基　　金：北京市自然科学基金资助项目(7202077);国家自然科学基金资助项目(82071438);北京市百千万人才工程资助项目(2017002);北京老年医院老年医学科研专项(2020bjlnyy-自-3)。

摘　　要：目的 探讨运动神经元病致病基因Dctn1编码蛋白p150glued对小鼠脊髓运动神经元中N-甲基-D-天冬氨酸(NMDA)受体的影响。方法 利用蛋白印迹法检测p150glued缺失(cKO)小鼠及对照(Ctrl)小鼠脊髓中NMDA受体亚基NR1和NR2B的含量。利用免疫荧光染色法观察Ctrl和cKO小鼠脊髓运动神经元中NRI分布的变化。结果 与Ctrl小鼠相比,6月龄cKO小鼠脊髓组织中NR1和NR2B的蛋白水平明显增加,cKO小鼠脊髓运动神经元中NR1阳性斑点数也明显增多、增大。结论 神经元中p150glued缺失可以引起6月龄小鼠脊髓运动神经元中NMDA受体亚基NR1和NR2B增多。Objective To investigate the regulation of motor neuron disease associated protein p150glued on the levels of N-methyl-D-aspartate(NMDA) receptor in spinal motor neurons of mice.Methods Western blot was applied to detect the levels of NR 1 and NR2B(the subunits of NMDA receptors) in the spinal cord of ctrl and cKO mice.The distribution of NR1 was observed by using immunofluorescence staining in the spinal motor neurons of ctrl and cKO mice.Results The levels of NR1 and NR2B were increased in the spinal cord of cKO mice.Compared with the ctrl mice,the number and size of NR1 puncta were increased in the spinal motor neurons of cKO mice.Conclusion Loss-of-function of p150glued resulted in the increase of NMDA receptor subunits(NR1 and NR2B) in the spinal motor neurons of mice.

关 键 词：运动神经元病 p150glued NMDA受体 脊髓运动神经元 

分 类 号：R744[医药卫生—神经病学与精神病学]