碱提酸沉法提取补骨脂中补骨脂乙素的工艺研究  

Study on the extraction process of isobavachalcone from Psoraleae corylifolia using alkaline extraction and acid precipitation

作　　者：孙小龙 戴轶群 马慧 俞俊霞 李红梅 朱美林 吴成柱 SUN Xiao-long;DAI Yi-qun;MA Hui;YU Jun-xia;LI Hong-mei;ZHU Mei-lin;WU Cheng-zhu(School of Pharmacy,Bengbu Medical College,Bengbu Anhui 233030,China)

机构地区：[1]蚌埠医学院药学院,安徽蚌埠233030

出　　处：《蚌埠医学院学报》2023年第5期656-660,共5页Journal of Bengbu Medical College

基　　金：安徽省自然科学基金(2008085MH284);安徽高校自然科学研究项目(KJ2019A0335);蚌埠医学院研究生科研创新计划项目(Byycxz20027)。

摘　　要：目的:通过碱提酸沉的方法对补骨脂乙素的提取进行工艺优化。方法:以补骨脂乙素的含量为指标,通过单因素试验和正交试验考察料液比、碱液浓度、碱提时间、酸沉pH、酸沉时间以及超声时间对补骨脂乙素提取工艺的影响。结果:碱提酸沉法提取补骨脂乙素的最佳工艺为:料液比为1∶10、碱液浓度为4%、碱提时间为2 h、酸沉pH为6、酸沉时间为0.5 h、超声时间为20 min。结论:本研究优化的最佳工艺条件具有良好的复重性,且稳定可靠、经济环保,适合大量提取补骨脂中的补骨脂乙素。Objective:To optimize the extraction process of isobavachalcone by alkaline extraction and acid precipitation.Methods:The content of isobavachalcone was set as the objective parameter.The effects of solid-liquid ratio,alkali concentration,alkali extraction time,acid precipitation pH,acid precipitation time and ultrasound time on extraction process of isobavachalcone were investigated through single factor test and orthogonal test.Results:The optimal extraction conditions of isobavachalcone by alkaline extraction and acid precipitation were as follows:the ratio of solid-liquid for 1∶10,alkali concentration for 4%,alkali extraction time for 2 h,acid precipitation pH 6,acid precipitation time for 0.5 h and ultrasound time for 20 min.Conclusions:In this study,the optimal process conditions have the good repeatability,and it is stable,reliable,economical and environmental-friendly,which can greatly improve the extraction rate of isobavachalcone from Psoralea corylifolia.

关 键 词：碱提酸沉法 补骨脂乙素 正交试验 高效液相色谱法 

分 类 号：R284.2[医药卫生—中药学]