胸腹水细胞块切片结合免疫组化在病理诊断中的应用  

Application of pleural and ascites cell block section combined with immunohistochemistry in pathological diagnosis

作　　者：刘艳 LIU Yan(Department of Pathology,Dandong Hospital of Traditional Chinese Medicine,Dandong 118000,China)

机构地区：[1]丹东市中医院病理科,118000

出　　处：《中国现代药物应用》2023年第10期71-73,共3页Chinese Journal of Modern Drug Application

摘　　要：目的 分析病理诊断中应用胸腹水细胞块切片+免疫组化的诊断价值。方法 200份临床送检胸腹水标本,均进行细胞块切片结合免疫组化、苏木精-伊红染色法(HE)检测。分析标本病理结果与检测阳性率,比较腺癌与恶性间皮瘤细胞角质蛋白7(CK7)、癌胚抗原(CEA)、Wilm’s肿瘤基因-1(WT-1)、钙结合蛋白(CR)抗体表达阳性情况。结果 200份临床送检胸腹水标本中,有40份疑似肿瘤性胸腹腔积液标本,病理诊断均为肿瘤性胸腹腔积液。细胞块切片结合免疫组化检测阳性率100.00%(40/40)高于HE染色检测的67.50%(27/40),差异有统计学意义(P<0.05)。腺癌CK7抗体表达阳性率100.00%、CEA抗体表达阳性率95.46%高于恶性间皮瘤的0、0,CR抗体表达阳性率4.54%低于恶性间皮瘤的75.00%,差异有统计学意义(P<0.05)。腺癌与恶性间皮瘤WT-1抗体表达阳性率比较,差异无统计学意义(P>0.05)。结论 胸腹水细胞块切片结合免疫组化临床病理诊断肿瘤细胞的阳性率高,有助于鉴别原发疾病类型、早期治疗。Objective To analyze the diagnostic value of pleural and ascites cell block section combined with immunohistochemistry in pathological diagnosis.Methods 200 specimens of pleural and ascites were tested by cell block sectioning combined with immunohistochemistry and hematoxylin-eosin staining(HE),and pathological results and positive detection were analyzed.The positive expression of cytokeratin 7(CK7),carcinoembryonic antigen(CEA),Wilm's tumor gene-1(WT-1)and calretinin(CR)antibodies in adenocarcinoma and malignant mesothelioma cells were compared.Results 200 specimens of pleural and ascites were submitted for clinical examination,including 40 specimens of suspected tumorous pleural effusion,and all were pathologically diagnosed as tumorous pleural effusion;the positive detection rate of cell block section combined with immunohistochemistry was 100.00%(40/40),which was higher than that of 67.50%(27/40)detected by HE staining,and the difference was statistically significant(P<0.05).The positive rates of antibody expression of CK7 and CEA in adenocarcinoma were 100.00%and 95.46%,which was higher than those of 0 and 0 in malignant mesothelioma;the positive rate of CR antibody expression was 4.54%,which was lower than that of 75.00%of malignant mesothelioma;the differences were statistically significant(P<0.05).There was no statistically significant difference in the positive rate of WT-1 antibody expression between adenocarcinoma and malignant mesothelioma(P>0.05).Conclusion The combination of pleural and ascites cell block section and immunohistochemical clinical pathological diagnosis of tumor cells has a high positive rate,which helps to identify the type of primary disease and early treatment.

关 键 词：胸腹水细胞块切片 免疫组化 病理诊断 苏木精-伊红染色法 抗体表达阳性 

分 类 号：R36[医药卫生—病理学]